木材腐烂真菌(WDF)是专门降解木材的真菌。一个有趣的观点是它们用作新型食品或食品成分的来源。这里,第一次,来自A.biennis的水醇和有机提取物的代谢物谱,F.伊贝丽卡,通过NMR方法研究了陆生菌丝体。氨基酸(丙氨酸,精氨酸天冬酰胺,天冬氨酸,甜菜碱,GABA,谷氨酸,谷氨酰胺,组氨酸,异亮氨酸,亮氨酸,赖氨酸,苯丙氨酸,苏氨酸,色氨酸,酪氨酸,缬氨酸),糖(半乳糖,葡萄糖,麦芽糖,海藻糖,甘露醇),有机酸(乙酸,柠檬酸盐甲酸盐,富马酸盐,乳酸,苹果酸,琥珀酸盐),腺苷,胆碱,在水醇提取物中鉴定和定量尿嘧啶和尿苷,而有机提取物的1H光谱显示存在饱和,单不饱和和二不饱和脂肪链,麦角固醇,1,2-二酰基-sn-甘油-3-磷脂酰乙醇胺,和1,2-二酰基-sasgamcilo-3-磷脂酰胆碱。双叶酵母提取物显示出最高的氨基酸浓度。一些化合物仅在特定物种中检测到:甜菜碱和甘露醇。A.biennis中的麦芽糖,伊贝里卡的半乳糖,在F.iberica和S.hirsutum的GABA,和乙酸盐在A.biennis和S.hirsutum。美国陆生显示出最高的饱和脂肪链浓度,而DUFA在A.biennis中达到最高浓度。在双歧杆菌和伊贝里卡中都测量到大量的麦角甾醇。报告的结果可用于开发具有高营养和营养保健价值的基于WDF的产品。
Wood Decay Fungi (WDF) are fungi specialized in degrading wood. An interesting perspective is their use as a source of Novel Foods or food ingredients. Here, for the first time, the metabolite profiling of hydroalcoholic and organic extracts from A. biennis, F. iberica, S. hirsutum
mycelia was investigated by NMR methodology. Amino acids (alanine, arginine, asparagine, aspartate, betaine, GABA, glutamate, glutamine, histidine, isoleucine, leucine, lysine, phenylalanine, threonine, tryptophan, tyrosine, valine), sugars (galactose, glucose, maltose, trehalose, mannitol), organic acids (acetate, citrate, formate, fumarate, lactate, malate, succinate), adenosine, choline, uracil and uridine were identified and quantified in the hydroalcoholic extracts, whereas the 1H spectra of organic extracts showed the presence of saturated, mono-unsaturated and di-unsaturated fatty chains, ergosterol,1,2-diacyl-sn-glycero-3-phosphatidylethanolamine, and 1,2-diacyl-sasglycero-3-phosphatidylcholine. A. biennis extracts showed the highest amino acid concentration. Some compounds were detected only in specific species: betaine and mannitol in S. hirsutum, maltose in A. biennis, galactose in F. iberica, GABA in F. iberica and S. hirsutum, and acetate in A. biennis and S. hirsutum. S. hirsutum showed the highest saturated fatty chain concentration, whereas DUFA reached the highest concentration in A. biennis. A high amount of ergosterol was measured both in A. biennis and F. iberica. The reported results can be useful in the development of WDF-based products with a high nutritional and nutraceutical value.