Abstract：
RNA double-strand hybridization is a key player in gene expression regulation. Single-stranded RNA of up to 300 nucleotides forms Watson-Crick base pairs with complementary messenger RNA. Fluorescence-based single-molecule methods allow to study RNA-RNA interaction under physiological conditions. Here is described, how the dissociation constant of RNA double strands can be determined by applying fluorescence correlation spectroscopy.
摘要：
RNA双链杂交是基因表达调控的关键角色。多达300个核苷酸的单链RNA与互补信使RNA形成沃森-克里克碱基对。基于荧光的单分子方法允许研究生理条件下的RNA-RNA相互作用。这里描述，如何通过应用荧光相关光谱法确定RNA双链的解离常数。
