%0 Journal Article
%T RNA Double-Helix Hybridization Measured by Fluorescence Correlation Spectroscopy.
%A Werner A
%J Methods Mol Biol
%V 2741
%N 0
%D 2024
%M 38217653
暂无%R 10.1007/978-1-0716-3565-0_9
%X RNA double-strand hybridization is a key player in gene expression regulation. Single-stranded RNA of up to 300 nucleotides forms Watson-Crick base pairs with complementary messenger RNA. Fluorescence-based single-molecule methods allow to study RNA-RNA interaction under physiological conditions. Here is described, how the dissociation constant of RNA double strands can be determined by applying fluorescence correlation spectroscopy.