{Reference Type}: Journal Article
{Title}: RNA Double-Helix Hybridization Measured by Fluorescence Correlation Spectroscopy.
{Author}: Werner A;
{Journal}: Methods Mol Biol
{Volume}: 2741
{Issue}: 0
{Year}: 2024
暂无{DOI}: 10.1007/978-1-0716-3565-0_9
{Abstract}: RNA double-strand hybridization is a key player in gene expression regulation. Single-stranded RNA of up to 300 nucleotides forms Watson-Crick base pairs with complementary messenger RNA. Fluorescence-based single-molecule methods allow to study RNA-RNA interaction under physiological conditions. Here is described, how the dissociation constant of RNA double strands can be determined by applying fluorescence correlation spectroscopy.