PDF(Pubmed)
Abstract：
Infertility affects millions worldwide, posing a significant global health challenge. The proteomic analysis of follicular fluid provides a comprehensive view of the complex molecular landscape within ovarian follicles, offering valuable information on the factors influencing oocyte development and on the overall reproductive health. The follicular fluid is derived from the plasma and contains various proteins that can have different roles in oocyte health and infertility, and this fluid is a critical microenvironment for the developing oocytes as well. Using the high-performance liquid chromatography-mass spectrometry method, we investigated the protein composition of the follicular fluid, and after classification, we carried out relative quantification of the identified proteins in the pregnant (P) and non-pregnant (NP) groups. Based on the protein-protein interaction analysis, albumin and apolipoprotein A1 (ApoA1) were found to be hub proteins, and the quantitative comparison of the P and NP groups resulted in a significantly lower concentration of ApoA1 and high-density lipoprotein cholesterol in the P group. As both molecules are involved in the cholesterol transport, we also investigated their role in the development of oocytes and in the prediction of fertility.
摘要：
不孕症影响着全世界数百万人，构成了重大的全球卫生挑战。卵泡液的蛋白质组学分析提供了卵巢卵泡内复杂分子景观的全面视图，提供有关影响卵母细胞发育的因素和整体生殖健康的有价值的信息。卵泡液来自血浆，含有各种蛋白质，这些蛋白质在卵母细胞健康和不孕症中起着不同的作用，这种液体也是发育中卵母细胞的关键微环境。采用高效液相色谱-质谱法,我们调查了卵泡液的蛋白质组成，分类后，我们对妊娠（P）和非妊娠（NP）组中鉴定的蛋白质进行了相对定量。基于蛋白质-蛋白质相互作用分析，白蛋白和载脂蛋白A1（ApoA1）被发现是枢纽蛋白，而P组和NP组的定量比较导致P组ApoA1和高密度脂蛋白胆固醇的浓度明显降低。因为这两个分子都参与了胆固醇的运输，我们还研究了它们在卵母细胞发育和生育力预测中的作用。
