背景:脂质,包括磷脂和胆汁酸,发挥各种信号作用,被认为有助于冠状动脉疾病(CAD)的发展。这里,我们的目的是比较冠心病患者和非冠心病患者血液中按性别分层的脂质组学和胆汁酸谱.
方法:从2015年到2022年,在INTERCATH队列中招募了3,012例接受冠状动脉造影的患者。从整个队列中,使用患者特征定义亚组,如CAD与没有CAD,1stvs.LDL-c的第三个三元组,和女性vs.男性。此后,基于年龄的匹配算法,BMI,高血压状态,糖尿病状态,吸烟状况,地中海饮食得分,和他汀类药物的摄入量,甘油三酯,HDL-c和hs-CRP的比例为1:1。使用Lipidyzer平台(SCIEX)对储存的血液样品进行脂质组学分析,并使用液相色谱和串联质谱(LC-MS/MS)进行胆汁酸分析。
结果:共分析了177名匹配个体;女性和男性CAD患者的平均年龄分别为73.5岁(第25和第75百分位数:64.1、78.2)和71.9岁(65.7、77.2)。分别,无CAD的女性和男性为67.6岁(58.3、75.3)和69.2岁(59.8、76.8),分别。进一步的基线特征,包括心血管危险因素,在群体之间保持平衡。女性冠心病患者的磷脂酰胆碱和二酰甘油水平下降,与没有CAD的女性患者相比,未检测到胆汁酸谱的差异。相比之下,在男性CAD患者中,次级胆汁酸种类糖脂胆酸和石胆酸的浓度降低,以及特定脂质水平的改变,与没有CAD的男性相比,被检测到。值得注意的是,低LDL-c和CAD的男性患者的各种磷脂种类浓度明显更高,特别是疟原虫,与高LDL-c亚组相比。
结论:我们提出了关于CAD患者性别特异性脂质组学模式和胆汁酸谱的假设生成数据。数据表明,改变的脂质和胆汁酸组成可能有助于CAD的发展和/或进展,帮助了解女性和男性CAD的不同疾病轨迹。
背景:https://clinicaltrials.gov/ct2/show/NCT04936438,唯一标识符:NCT04936438。
BACKGROUND: Lipids, including phospholipids and bile acids, exert various signaling effects and are thought to contribute to the development of coronary artery disease (CAD). Here, we aimed to compare lipidomic and bile acid profiles in the blood of patients with and without CAD stratified by sex.
METHODS: From 2015 to 2022, 3,012 patients who underwent coronary angiography were recruited in the INTERCATH cohort. From the overall cohort, subgroups were defined using patient characteristics such as CAD vs. no CAD, 1st vs. 3rd tertile of LDL-c, and female vs. male sex. Hereafter, a matching algorithm based on age, BMI, hypertension status, diabetes mellitus status, smoking status, the Mediterranean diet score, and the intake of statins, triglycerides, HDL-c and hs-CRP in a 1:1 ratio was implemented. Lipidomic analyses of stored blood samples using the Lipidyzer platform (SCIEX) and bile acid analysis using liquid chromatography with tandem mass spectrometry (LC‒MS/MS) were carried out.
RESULTS: A total of 177 matched individuals were analyzed; the median ages were 73.5 years (25th and 75th percentile: 64.1, 78.2) and 71.9 years (65.7, 77.2) for females and males with CAD, respectively, and 67.6 years (58.3, 75.3) and 69.2 years (59.8, 76.8) for females and males without CAD, respectively. Further baseline characteristics, including cardiovascular risk factors, were balanced between the groups. Women with CAD had decreased levels of phosphatidylcholine and diacylglycerol, while no differences in bile acid profiles were detected in comparison to those of female patients without CAD. In contrast, in male patients with CAD, decreased concentrations of the secondary bile acid species glycolithocholic and lithocholic acid, as well as altered levels of specific lipids, were detected compared to those in males without CAD. Notably, male patients with low LDL-c and CAD had significantly greater concentrations of various phospholipid species, particularly plasmalogens, compared to those in high LDL-c subgroup.
CONCLUSIONS: We present hypothesis-generating data on sex-specific lipidomic patterns and bile acid profiles in CAD patients. The data suggest that altered lipid and bile acid composition might contribute to CAD development and/or progression, helping to understand the different disease trajectories of CAD in women and men.
BACKGROUND: https://clinicaltrials.gov/ct2/show/NCT04936438 , Unique identifier: NCT04936438.