PDF(Pubmed)
Abstract：
The endemic chub Squalius tenellus (Heckel, 1843) was introduced more than 100 years ago to Lake Blidinje (Bosnia-Herzegovina). Only 1 species of enteric helminth was found in a sample of 35 chubs, the tapeworm Caryophyllaeus brachycollis (Janiszewska, 1953). The paper includes histopathological investigation with identification of innate immune cells involved in host reaction and molecular data allowed correct designation of the cestode species. Of 35 specimens of chub examined, 21 (60%) harboured individuals of C. brachycollis and a total of 1619 tapeworms were counted, the intensity of infection ranged from 1 to 390 worms per fish (46.2 ± 15.3, mean ± s.e.). Histopathological and ultrastructural investigations showed strict contact between the worm\'s body and the epithelia and increase in the number of mucous cells, rodlet cells among the epithelial cells. Within the tunica propria-submucosa, beneath the site of scolex attachment, numerous neutrophils and mast cells were noticed. This is the first study of the occurrence of C. brachycollis in chub from Lake Blidinje and on the response of the innate immune cells of S. tenellus to this tapeworm. Interestingly, in 3 very heavily infected chubs, perforation of the intestinal wall was documented; this is uncommon among cestodes which use fish as a definitive host.
摘要：
特有的ChubSquialustenellus（Heckel，1843年）是在100多年前引入Blidinje湖（波斯尼亚和黑塞哥维那）的。在35个chubs的样本中只发现了1种肠蠕虫，tape虫Caryophyllaeusbrachycollis（Janiszewska，1953).本文包括组织病理学研究，其中包括鉴定参与宿主反应的先天免疫细胞，以及允许正确指定c虫物种的分子数据。在检查的35个样本中，有21例(60%)藏有短尾梭菌,共1619只,感染强度为每条鱼1至390只蠕虫（46.2±15.3，平均值±s.e.）。组织病理学和超微结构研究表明，蠕虫的身体和上皮之间有严格的接触，粘液细胞的数量增加，上皮细胞中的Rodlet细胞。在固有膜粘膜下层，在scolex附件的下面，观察到大量中性粒细胞和肥大细胞。这是对来自Blidinje湖的chub中发生的C.brachycollis的发生以及对S.tenellus的先天性免疫细胞对这种tenellus虫的反应的首次研究。有趣的是,在三个感染严重的病人中，记录了肠壁穿孔；这在使用鱼作为最终宿主的c虫中并不常见。
