背景:种子老化,储存过程中发生的自然和不可避免的过程。燕麦,一种一年生草本植物,属于Gramineae家族和pooideae。除了是一种健康的食物,燕麦作为生态牧场,防治土壤盐渍化和荒漠化。它们还在促进草地农业和补充冬季牲畜饲料方面发挥作用。然而,燕麦种子的高脂和脂肪衍生物含量使它们容易变质,因为脂肪衍生物容易酸败,影响燕麦种子生产,storage,发展,和种质资源利用。关于衰老对覆盖和裸露燕麦种子的生理和细胞学结构影响的比较研究有限。因此,我们的研究旨在确定人工老化的种子变质机制。3\'(A.sativa)和\'白岩号。2\'(A.努达)种子,为燕麦种子老化的生理评价提供依据,为燕麦的科学安全贮藏和高效利用提供参考。
结果:在两个燕麦品种中,种子中的超氧化物歧化酶和过氧化氢酶活性呈上升和下降趋势,分别。方差分析显示,在不同的老化时间,两个品种之间燕麦种子的所有测量指标均存在显着差异和交互作用。\'龙岩号3\'种子,年龄为24-96小时,发芽率<30%,电导率,丙二醛,可溶性糖,和可溶性蛋白质水平的增加比“白岩号”更显著。2\'.随着长期老化导致细胞膜降解,活性氧积累,破坏抗氧化酶系统,明显的胚胎细胞肿胀,和无序的细胞排列,阻断营养供应途径.同时,染色质在细胞核中严重集中,线粒体结构受损,并注意到能量代谢受损,导致\'龙岩编号的损失。3\'种子的生命力和价值。相反,\'白岩号.2种子老化96小时后发芽率为73.33%,在衰老过程中持续较高的抗氧化酶活性,正常的胚胎细胞形状,和内质网的存在。
结论:老化燕麦种子的ROS积累和抗氧化酶系统损伤,核染色质凝聚,线粒体结构损伤,核酸代谢和呼吸减弱,燕麦种子活力下降。\'龙岩号3'种子在人工老化下的破坏比'白岩号更严重。2\'种子,突出了它们对衰老影响的高度敏感性。
BACKGROUND: Seed aging, a natural and inevitable process occurring during storage. Oats, an annual herb belonging to the Gramineae family and pooideae. In addition to being a healthy food, oats serve as ecological pastures, combating soil salinization and desertification. They also play a role in promoting grassland agriculture and supplementing winter livestock feed. However, the high lipid and fat derivatives contents of oat seeds make them susceptible to deterioration, as fat derivatives are prone to rancidity, affecting oat seed production, storage, development, and germplasm resource utilization. Comparative studies on the effects of aging on physiology and cytological structure in covered and naked oat seeds are limited. Thus, our study aimed to determine the mechanism underlying seed deterioration in artificially aged \'LongYan No. 3\' (A. sativa) and \'BaiYan No. 2\' (A. nuda) seeds, providing a basis for the physiological evaluation of oat seed aging and serving as a reference for scientifically safe storage and efficient utilization of oats.
RESULTS: In both oat varieties, superoxide dismutase and catalase activities in seeds showed increasing and decreasing trends, respectively. Variance analysis revealed significant differences and interaction in all measured indicators of oat seeds between the two varieties at different aging times. \'LongYan No. 3\' seeds, aged for 24-96 h, exhibited a germination rate of < 30%, Conductivity, malondialdehyde, soluble sugar, and soluble protein levels increased more significantly than the \'BaiYan No. 2\'. With prolonged aging leading to cell membrane degradation, reactive oxygen species accumulation, disrupted antioxidant enzyme system, evident embryo cell swelling, and disordered cell arrangement, blocking the nutrient supply route. Simultaneously, severely concentrated chromatin in the nucleus, damaged mitochondrial structure, and impaired energy metabolism were noted, resulting in the loss of \'LongYan No. 3\' seed vitality and value. Conversely, \'BaiYan No. 2\' seeds showed a germination rate of 73.33% after 96 h of aging, consistently higher antioxidant enzyme activity during aging, normal embryonic cell shape, and existence of the endoplasmic reticulum.
CONCLUSIONS: ROS accumulation and antioxidant enzyme system damage in aged oat seeds, nuclear chromatin condensation, mitochondrial structure damage, nucleic acid metabolism and respiration weakened, oat seed vigor decreased. \'LongYan No. 3\' seeds were more severely damaged under artificial aging than \'BaiYan No. 2\' seeds, highlighting their heightened susceptibility to aging effects.