Abstract：
Rhizoctonia solani endornavirus 8 (RsEV8) was isolated from strain XY175 of Rhizoctonia solani AG-1 IA. The full-length genome of RsEV8 is 16,147 nucleotides (nt) in length and contains a single open reading frame that encodes a large polyprotein of 5227 amino acids. The polyprotein contains four conserved domains: viral methyltransferase, putative DEAH box helicase, viral helicase, and RNA-dependent RNA polymerase (RdRp). RsEV8 has a shorter 3\'-UTR (58 nt) and a longer 5\'-UTR (404 nt). A multiple sequence alignment indicated that the RdRp of RsEV8 possesses eight typical RdRp motifs. According to a BLASTp analysis, RsEV8 shares 39.31% sequence identity with Rhizoctonia cerealis endornavirus-1084-7. Phylogenetic analysis demonstrated that RsEV8 clusters with members of the genus Betaendornavirus.
摘要：
从solanirhizoctoniaAG-1IA的XY175株中分离出solanirhizoctoniaendernnavirus8（RsEV8）。RsEV8的全长基因组长度为16,147个核苷酸(nt),并且包含编码5227个氨基酸的大的多蛋白的单个开放阅读框。多蛋白包含四个保守域：病毒甲基转移酶，推定的DEAH盒解旋酶,病毒解旋酶,和RNA依赖性RNA聚合酶(RdRp)。RsEV8具有较短的3'-UTR(58nt)和较长的5'-UTR(404nt)。多序列比对表明RsEV8的RdRp具有八个典型的RdRp基序。根据BLASTp分析，RsEV8与谷物内甲病毒-1084-7具有39.31%的序列同一性。系统发育分析表明，RsEV8与Betaendernna病毒属的成员聚集在一起。
