%0 Journal Article
%T Characterization of a novel endornavirus isolated from the phytopathogenic fungus Rhizoctonia solani.
%A Li Y
%A Huang X
%A Zhou G
%A Ye A
%A Deng Y
%A Shi L
%A Zhang R
%J Arch Virol
%V 169
%N 1
%D 2024 Jan 2
%M 38163823
%F 2.685
%R 10.1007/s00705-023-05915-2
%X Rhizoctonia solani endornavirus 8 (RsEV8) was isolated from strain XY175 of Rhizoctonia solani AG-1 IA. The full-length genome of RsEV8 is 16,147 nucleotides (nt) in length and contains a single open reading frame that encodes a large polyprotein of 5227 amino acids. The polyprotein contains four conserved domains: viral methyltransferase, putative DEAH box helicase, viral helicase, and RNA-dependent RNA polymerase (RdRp). RsEV8 has a shorter 3'-UTR (58 nt) and a longer 5'-UTR (404 nt). A multiple sequence alignment indicated that the RdRp of RsEV8 possesses eight typical RdRp motifs. According to a BLASTp analysis, RsEV8 shares 39.31% sequence identity with Rhizoctonia cerealis endornavirus-1084-7. Phylogenetic analysis demonstrated that RsEV8 clusters with members of the genus Betaendornavirus.