{Reference Type}: Journal Article
{Title}: Characterization of a novel endornavirus isolated from the phytopathogenic fungus Rhizoctonia solani.
{Author}: Li Y;Huang X;Zhou G;Ye A;Deng Y;Shi L;Zhang R;
{Journal}: Arch Virol
{Volume}: 169
{Issue}: 1
{Year}: 2024 Jan 2
{Factor}: 2.685
{DOI}: 10.1007/s00705-023-05915-2
{Abstract}: Rhizoctonia solani endornavirus 8 (RsEV8) was isolated from strain XY175 of Rhizoctonia solani AG-1 IA. The full-length genome of RsEV8 is 16,147 nucleotides (nt) in length and contains a single open reading frame that encodes a large polyprotein of 5227 amino acids. The polyprotein contains four conserved domains: viral methyltransferase, putative DEAH box helicase, viral helicase, and RNA-dependent RNA polymerase (RdRp). RsEV8 has a shorter 3'-UTR (58 nt) and a longer 5'-UTR (404 nt). A multiple sequence alignment indicated that the RdRp of RsEV8 possesses eight typical RdRp motifs. According to a BLASTp analysis, RsEV8 shares 39.31% sequence identity with Rhizoctonia cerealis endornavirus-1084-7. Phylogenetic analysis demonstrated that RsEV8 clusters with members of the genus Betaendornavirus.