Abstract：
Full-thickness cutaneous trauma, due to the lack of dermis, leads to difficulty in epithelialization by keratinocytes, developing a fibrotic scar, with less elasticity than the original skin, which may have disorders in predisposed individuals, resulting in hypertrophic scar and keloids. Biomedical materials have excellent characteristics, such as good biocompatibility and low immunogenicity, which can temporarily replace traditional materials used as primary dressings. In this work, we developed two dermal matrices based on Nile tilapia collagen, with (M_GAG) and without (M) glycosaminoglycans, using a sugarcane polymer membrane as a matrix support. To assess the molecular mechanisms driving wound healing, we performed qualitative proteomic analysis on the wound bed in an in vivo study involving immunocompetent murine models at 14 and 21 days post-full-thickness skin injury. Gene Ontology and Pathway analysis revealed that both skins were markedly represented by modulation of the immune system, emphasizing controlling the acute inflammation response at 14 and 21 days post-injury. Furthermore, both groups showed significant enrichment of pathways related to RNA and protein metabolism, suggesting an increase in protein synthesis required for tissue repair and proper wound closure. Other pathways, such as keratinization and vitamin D3 metabolism, were also enriched in the groups treated with M matrix. Finally, both matrices improved wound healing in a full post-thick skin lesion. However, our preliminary molecular data reveals that the collagen-mediated healing matrix lacking glycosaminoglycan (M) exhibited a phenotype more favorable to tissue repair, making it more suitable for use before skin grafts.
摘要：
全层皮肤创伤,由于缺乏真皮，导致角质形成细胞的上皮化困难，形成纤维化疤痕，弹性比原始皮肤小，可能在易感个体中出现疾病，导致增生性瘢痕和瘢痕疙瘩.生物医学材料具有优异的特性,如良好的生物相容性和低免疫原性，可以暂时取代用作主要敷料的传统材料。在这项工作中,我们开发了两种基于尼罗罗非鱼胶原蛋白的真皮基质，含(M_GAG)和不含(M)糖胺聚糖，使用甘蔗聚合物膜作为基质载体。评估驱动伤口愈合的分子机制，我们在全厚度皮肤损伤后14天和21天的一项涉及免疫活性小鼠模型的体内研究中，对伤口床进行了定性蛋白质组学分析。基因本体论和通路分析显示，两种皮肤都明显表现为免疫系统的调节，强调在损伤后14天和21天控制急性炎症反应。此外，两组都显示出与RNA和蛋白质代谢相关的途径的显着富集，提示组织修复和适当伤口闭合所需的蛋白质合成增加。其他途径,如角质化和维生素D3代谢，在用M基质处理的组中也富集。最后,两种基质均可改善全厚后皮肤病变的伤口愈合。然而,我们的初步分子数据表明，缺乏糖胺聚糖（M）的胶原介导的愈合基质表现出更有利于组织修复的表型，使其更适合皮肤移植前使用。
