益生菌作为水产养殖中的环保替代品越来越受欢迎。然而,关于它们对红罗非鱼(OreochromisniloticusxO.mosambicus)亲鱼繁殖效率的影响的研究有限。因此,本实验旨在探讨选择性益生菌枯草芽孢杆菌和地衣芽孢杆菌(BSL;1:1)对血液血液学的联合作用,血清代谢物,性腺组织学,繁殖性能,和红罗非鱼亲鱼的生殖相关基因。在四个处理组中放养重140-160克的罗非鱼亲鱼:对照组(T0),其他三组分别向水中添加不同水平的BSL,如下所示:T1(0.01g/m3),T2(0.02g/m3),和T3(0.03g/m3),分别。结果表明,BSL管理显着改善红细胞,血红蛋白,血细胞比容,MCH,和MCHC,T3组改善程度最高(P<0.05)。添加到鱼水中的BSL显着增强了血清蛋白组分(总蛋白,白蛋白,和球蛋白),而AST,ALT,ALP,肌酐,尿酸,血糖呈剂量依赖性下降(P<0.05)。与其他组相比,添加0.02-0.03g/m3的BSL导致更高的抗氧化状态(超氧化物歧化酶和过氧化氢酶)(P<0.05)。T3组睾酮水平高于其他组(P<0.05)。所有女性荷尔蒙(LH,FSH,雌二醇,和孕酮)通过添加BSL而大大增加。此外,BSL组表现出更高的GSI,HSI,VSI(仅限男性),鸡蛋直径(mm),鱼苗/鱼的平均数量,和平均鱼苗重量(g)与对照组相比(P<0.05)。生殖相关基因的表达(vasa,nanos1a,nanos2,dnd1,pum1,AMH,和vtg)在0.03g/m3处理中鱼的性腺中显着上调。组织学性腺结构表明,BSL可改善两种罗非鱼的性腺成熟。总的来说,添加枯草芽孢杆菌和地衣芽孢杆菌的混合物(0.03g/m3水)可以通过上调生殖基因来加速红罗非鱼的繁殖性能并增强健康状况。
Probiotics are becoming increasingly popular as eco-friendly alternatives in aquaculture. However, there is limited research on their impacts on the reproductive efficiency of Red
Tilapia (Oreochromis niloticus x O. mossambicus) broodstock. Therefore, this experiment aimed to explore the combined effects of selective probiotics Bacillus subtilis and B. licheniformis (BSL; 1:1) added to water on blood hematology, serum metabolites, gonadal histology, reproductive performance, and reproductive associated genes in Red
Tilapia broodstock.
Tilapia broodfish weighing 140-160 g were stocked in four treatment groups: control (T0), and the other three groups were added different levels of BSL to the water as follows: T1 (0.01 g/m3), T2 (0.02 g/m3), and T3 (0.03 g/m3), respectively. Results indicate that BSL administration significantly improved RBCs, hemoglobin, hematocrit, MCH, and MCHC, with the highest improvement seen in the T3 group (P < 0.05). BSL added to the fish water significantly enhanced serum protein fractions (total protein, albumin, and globulins), while AST, ALT, ALP, creatinine, uric acid, and glucose were significantly diminished in a dose-dependent way (P < 0.05). Adding 0.02-0.03 g/ m3 of BSL resulted in higher antioxidant status (superoxide dismutase and catalase) compared to other groups (P < 0.05). Testosterone levels were higher in T3 than in other groups (P < 0.05). All female hormones (LH, FSH, estradiol, and progesterone) were substantially augmented by the addition of BSL. Additionally, the BSL groups exhibited higher GSI, HSI, VSI (male only), egg diameter (mm), mean number of fry/fish, and mean fry weight (g) compared to the control group (P < 0.05). Expression of reproductive-associated genes (vasa, nanos1a, nanos2, dnd1, pum1, AMH, and vtg) were significantly up-regulated in the gonads of fish in the 0.03 g/m3 treatment. The histological gonadal structure exhibited that BSL improved gonad maturation in both genders of
Tilapia fish. Overall, adding a mixture of B. subtilis and B. licheniformis (0.03 g/m3 water) can accelerate reproductive performance in Red
Tilapia through up-regulation of reproductive genes and enhance the health profile.