PDF(Pubmed)
Abstract：
BACKGROUND: Shigella flexneri (S. flexneri) is a common intestinal pathogenic bacteria that mainly causes bacillary dysentery, especially in low socioeconomic countries. This study aimed to apply cold atmospheric plasma (CAP) on S. flexneri directly to achieve rapid, efficient and environmentally friendly sterilization.
METHODS: The operating parameters of the equipment were determined by plasma diagnostics. The plate count and transmission electron microscope were employed to calculate bacterial mortality rates and observe the morphological damage of bacterial cells. Measurement of intracellular reactive oxygen species (ROS) and superoxide anions were detected by 2,7-dichlorodihydrofluorescein (DCFH) and Dihydroethidium fluorescence probes, respectively. The fluorescence intensity (a. u.) reflects the relative contents. Additionally, the experiment about the single effect of temperature, ultraviolet (UV), and ROS on bacteria was conducted.
RESULTS: The peak discharge voltage and current during plasma operation were 3.92kV and 66mA. After discharge, the bacterial mortality rate of 10, 20, 30 and 40 s of plasma treatment was 60.71%, 74.02%, 88.11% and 98.76%, respectively. It was shown that the intracellular ROS content was proportional to the plasma treatment time and ROS was the major contributor to bacterial death.
CONCLUSIONS: In summary, our results illustrated that the plasma treatment could inactivate S. flexneri efficiently, and the ROS produced by plasma is the leading cause of bacterial mortality. This highly efficient sterilization method renders plasma a highly promising solution for hospitals, clinics, and daily life.
METHODS: The operating parameters of the equipment were determined by plasma diagnostics. The plate count and transmission electron microscope were employed to calculate bacterial mortality rates and observe the morphological damage of bacterial cells. Measurement of intracellular reactive oxygen species (ROS) and superoxide anions were detected by 2,7-dichlorodihydrofluorescein (DCFH) and Dihydroethidium fluorescence probes, respectively. The fluorescence intensity (a. u.) reflects the relative contents. Additionally, the experiment about the single effect of temperature, ultraviolet (UV), and ROS on bacteria was conducted.
RESULTS: The peak discharge voltage and current during plasma operation were 3.92kV and 66mA. After discharge, the bacterial mortality rate of 10, 20, 30 and 40 s of plasma treatment was 60.71%, 74.02%, 88.11% and 98.76%, respectively. It was shown that the intracellular ROS content was proportional to the plasma treatment time and ROS was the major contributor to bacterial death.
CONCLUSIONS: In summary, our results illustrated that the plasma treatment could inactivate S. flexneri efficiently, and the ROS produced by plasma is the leading cause of bacterial mortality. This highly efficient sterilization method renders plasma a highly promising solution for hospitals, clinics, and daily life.
摘要：
背景：福氏志贺氏菌（S.flexneri）是一种常见的肠道致病菌，主要引起细菌性痢疾，特别是在低社会经济国家。本研究旨在将冷大气等离子体(CAP)直接应用于福氏杆菌,高效环保杀菌。
方法：通过等离子体诊断确定设备的操作参数。平板计数和透射电子显微镜用于计算细菌死亡率并观察细菌细胞的形态损伤。通过2,7-二氯二氢荧光素（DCFH）和二氢乙锭荧光探针检测细胞内活性氧（ROS）和超氧阴离子，分别。荧光强度(a。u.)反映了相关内容。此外,关于温度单一效应的实验，紫外线(UV),并对细菌进行了ROS。
结果：等离子体操作过程中的峰值放电电压和电流分别为3.92kV和66mA。放电后,血浆处理10、20、30和40s的细菌死亡率为60.71％，74.02%,88.11%和98.76%，分别。结果表明，细胞内ROS含量与血浆处理时间成正比，ROS是细菌死亡的主要原因。
结论：总之，我们的结果表明，等离子体处理可以有效地灭活福氏杆菌，血浆产生的ROS是细菌死亡的主要原因。这种高效的灭菌方法使等离子体成为医院非常有前途的解决方案，诊所,和日常生活。
方法：通过等离子体诊断确定设备的操作参数。平板计数和透射电子显微镜用于计算细菌死亡率并观察细菌细胞的形态损伤。通过2,7-二氯二氢荧光素（DCFH）和二氢乙锭荧光探针检测细胞内活性氧（ROS）和超氧阴离子，分别。荧光强度(a。u.)反映了相关内容。此外,关于温度单一效应的实验，紫外线(UV),并对细菌进行了ROS。
结果：等离子体操作过程中的峰值放电电压和电流分别为3.92kV和66mA。放电后,血浆处理10、20、30和40s的细菌死亡率为60.71％，74.02%,88.11%和98.76%，分别。结果表明，细胞内ROS含量与血浆处理时间成正比，ROS是细菌死亡的主要原因。
结论：总之，我们的结果表明，等离子体处理可以有效地灭活福氏杆菌，血浆产生的ROS是细菌死亡的主要原因。这种高效的灭菌方法使等离子体成为医院非常有前途的解决方案，诊所,和日常生活。
