PDF(Pubmed)
Abstract：
This study investigated the decay rates of wastewater-associated markers and enteric viruses in laboratory microcosms mimicking estuarine water environments in temperate Sydney, NSW, Australia using qPCR and RT-qPCR assays. The results demonstrated the reduction in concentrations of Bacteroides HF183, Lachnospiraceae Lachno3, cross-assembly phage (crAssphage), pepper mild mottle virus (PMMoV), human adenovirus (HAdV 40/41), and enterovirus (EV) over a span of 42 days under spring/summer temperatures, presence/absence of microbiota, and different light conditions. The study found that HF183, Lachno3, crAssphage, PMMoV, HAdV 40/41, and EV exhibited varying decay rates depending on the experimental conditions. The average T90 values ranged from a few days to several months, indicating the rapid decay or prolonged persistence of these markers and enteric viruses in the estuarine environment. Furthermore, the study examined the effects of indigenous microbiota and spring/summer temperatures on wastewater-associated markers and enteric viruses decay rates. It was found that the presence of microbiota and temperature significantly influenced the decay rates of HF183 and PMMoV. Additionally, the study compared the effects of artificial sunlight and spring/summer temperatures on marker decay rates. Bacterial markers decayed faster than viral markers, although among viral markers crAssphage decay rates were relatively faster when compared to PMMoV. The exposure to artificial sunlight significantly accelerated the decay rates of bacterial markers, viral markers, and enteric viruses. Temperature also had an impact on the decay rates of Lachno3, crAssphage, and HAdV 40/41. In conclusion, this study provides valuable insights into the decay rates of wastewater-associated markers and enteric viruses under different experimental conditions that mimicked temperate environmental conditions. The findings contribute to our understanding of the fate and persistence of these markers in the environment which is crucial for assessing and managing risks from contamination by untreated human wastewater.
摘要：
这项研究调查了模拟悉尼温带河口水环境的实验室微观环境中与废水相关的标记物和肠道病毒的衰减率，新南威尔士州,澳大利亚使用qPCR和RT-qPCR测定。结果表明，拟杆菌HF183，LachnospileaeLachno3，交叉组装噬菌体（crAssphage）的浓度降低，辣椒轻度斑驳病毒（PMMoV），人腺病毒（HAdV40/41），和肠道病毒（EV）在春季/夏季温度下持续42天，微生物群,不同的光照条件研究发现，HF183，Lachno3，crAssphage，PMMoV,HAdV40/41和EV表现出不同的衰减率，具体取决于实验条件。平均T90值从几天到几个月不等，表明这些标记在河口环境中的快速衰减或长期持续存在。此外，该研究检查了本地微生物群和春季/夏季温度对废水相关标记和肠道病毒腐烂率的影响。发现微生物群和温度的存在显着影响HF183和PMMoV的衰减速率。此外,该研究比较了人工阳光和春季/夏季温度对标记衰变率的影响。细菌标记比病毒标记衰减更快，尽管与PMMoV相比，在病毒标记物中，crAssphage的衰减速率相对较快。暴露在人造阳光下显著加速了细菌标记物的腐烂率,病毒标记，HAdV,PMMoV,和EV。温度也影响了Lachno3，crAssphage的衰变率，和HAdV40/41。总之,这项研究为模拟温带环境条件的不同实验条件下废水相关标记和肠道病毒的衰减率提供了有价值的见解。这些发现有助于我们了解这些标记物在环境中的命运和持久性，这对于评估和管理废水污染的风险至关重要。
