PDF(Pubmed)
Abstract：
UNASSIGNED: To further investigate why curcumin (CUR) can attenuate psoriasis-like dermatitis of mice.
UNASSIGNED: Sixteen mice were randomized into four groups. The control group used carrier cream, and the model and the CUR group were applied with topical 5% imiquimod in the naked mice skin once a day for 6 days (62.5 mg/day/mice). Meanwhile, the control and model mice were given the same dose of saline by oral means, while mice in the CUR groups received oral drug doses of 50 and 100 mg/kg once a day for 6 days, respectively. CUR could largely improve imiquimod-induced lesions of mice. By using the ELISA and qPCR, we found that the protein and mRNA levels of epidermal TNF-α and IL-6 were inhibited by CUR. The phosphorylation levels of STAT3 and its downstream associated protein levels (eg, Cyclin D1, Bcl-2 and Pim1) in skin tissues of different groups were also inhibited by CUR. Furthermore, the results of immunohistochemistry also showed the repressed effect of CUR for the expression of TNF-α, IL-6 and p-STAT3 in psoriasis-like lesions of mice.
UNASSIGNED: CUR can effectively ameliorate the featured lesions of psoriasis mice, which may be closely associated with the involvement of IL-6/STAT3 signaling.
UNASSIGNED: Sixteen mice were randomized into four groups. The control group used carrier cream, and the model and the CUR group were applied with topical 5% imiquimod in the naked mice skin once a day for 6 days (62.5 mg/day/mice). Meanwhile, the control and model mice were given the same dose of saline by oral means, while mice in the CUR groups received oral drug doses of 50 and 100 mg/kg once a day for 6 days, respectively. CUR could largely improve imiquimod-induced lesions of mice. By using the ELISA and qPCR, we found that the protein and mRNA levels of epidermal TNF-α and IL-6 were inhibited by CUR. The phosphorylation levels of STAT3 and its downstream associated protein levels (eg, Cyclin D1, Bcl-2 and Pim1) in skin tissues of different groups were also inhibited by CUR. Furthermore, the results of immunohistochemistry also showed the repressed effect of CUR for the expression of TNF-α, IL-6 and p-STAT3 in psoriasis-like lesions of mice.
UNASSIGNED: CUR can effectively ameliorate the featured lesions of psoriasis mice, which may be closely associated with the involvement of IL-6/STAT3 signaling.
摘要：
■进一步研究为什么姜黄素（CUR）可以减轻小鼠的牛皮癣样皮炎。
■将16只小鼠随机分为4组。对照组使用载体乳膏，模型和CUR组在裸鼠皮肤中局部施用5%咪喹莫特，每天一次，共6天(62.5mg/天/小鼠)。同时,对照组和模型小鼠口服给予相同剂量的生理盐水，而CUR组中的小鼠每天一次接受50和100mg/kg的口服药物剂量，持续6天，分别。CUR可以在很大程度上改善咪喹莫特诱导的小鼠病变。通过使用ELISA和qPCR，我们发现表皮TNF-α和IL-6的蛋白和mRNA水平被CUR抑制。STAT3的磷酸化水平及其下游相关蛋白水平（例如，CUR也抑制了不同组皮肤组织中的CyclinD1，Bcl-2和Pim1）。此外，免疫组织化学结果还显示了CUR对TNF-α表达的抑制作用，IL-6和p-STAT3在小鼠银屑病样病变中的表达。
■CUR能有效改善银屑病小鼠的特征性病变，这可能与IL-6/STAT3信号传导的参与密切相关。
■将16只小鼠随机分为4组。对照组使用载体乳膏，模型和CUR组在裸鼠皮肤中局部施用5%咪喹莫特，每天一次，共6天(62.5mg/天/小鼠)。同时,对照组和模型小鼠口服给予相同剂量的生理盐水，而CUR组中的小鼠每天一次接受50和100mg/kg的口服药物剂量，持续6天，分别。CUR可以在很大程度上改善咪喹莫特诱导的小鼠病变。通过使用ELISA和qPCR，我们发现表皮TNF-α和IL-6的蛋白和mRNA水平被CUR抑制。STAT3的磷酸化水平及其下游相关蛋白水平（例如，CUR也抑制了不同组皮肤组织中的CyclinD1，Bcl-2和Pim1）。此外，免疫组织化学结果还显示了CUR对TNF-α表达的抑制作用，IL-6和p-STAT3在小鼠银屑病样病变中的表达。
■CUR能有效改善银屑病小鼠的特征性病变，这可能与IL-6/STAT3信号传导的参与密切相关。
