PDF(Pubmed)
Abstract：
Leptospirosis is a bacterial zoonotic disease. Humans and dogs are susceptible hosts, with similar clinical manifestations ranging from a febrile phase to multiple organ dysfunction. The incidence of leptospirosis in mainland France is relatively high, at about 1 case per 100,000 inhabitants, but our knowledge of the strains circulating in humans and dogs remains limited. We studied the polymorphism of the lfb1 gene sequences in an exhaustive database, to facilitate the identification of Leptospira strains. We identified 46 species-groups (SG) encompassing the eight pathogenic species of Leptospira. We sequenced the lfb1 gene amplification products from 170 biological samples collected from 2019 to 2021: 110 from humans and 60 from dogs. Epidemiological data, including vaccination status in dogs, were also collected. Three Leptospira species displaying considerable diversity were identified: L. interrogans, with eight lfb1 species-groups (including five new lfb1 species-groups) in humans and dogs; L. kirschneri, with two lfb1 species-groups in humans and dogs; and L. borgpetersenii, with one lfb1 species-group in humans only. The lfb1 species-group L. interrogans SG1, corresponding to serovar Icterohaemorrhagiae or Copenhageni, was frequently retrieved from both humans and dogs (n=67/110; 60.9% and n=59/60; 98.3% respectively). A high proportion of the affected dogs developed the disease despite vaccination (n=30/60; 50%). Genotyping with the polymorphic lfb1 gene is both robust and simple. This approach provided the first global picture of the Leptospira strains responsible for acute infections in mainland France, based on biological samples but without the need for culture. Identification of the Leptospira strains circulating and their changes over time will facilitate more precise epidemiological monitoring of susceptible and reservoir species. It should also facilitate the monitoring of environmental contamination, making it possible to implement preventive measures and to reduce the burden of this disease.
摘要：
钩端螺旋体病是一种细菌性人畜共患疾病。人类和狗是易感宿主，具有类似的临床表现，从发热期到多器官功能障碍。法国大陆的钩端螺旋体病发病率相对较高,每10万居民约有1例，但是我们对在人类和狗中传播的菌株的了解仍然有限。我们在详尽的数据库中研究了lfb1基因序列的多态性，便于钩端螺旋体菌株的鉴定。我们确定了46个物种组（SG），包括钩端螺旋体的8个致病物种。我们对2019年至2021年收集的170个生物样品的lfb1基因扩增产物进行了测序：110个来自人类，60个来自狗。流行病学数据,包括狗的疫苗接种情况，也被收集了。确定了三种表现出相当多样性的钩端螺旋体：L.rologans，人类和狗有八个lfb1物种群(包括五个新的lfb1物种群)；L.kirschneri，在人类和狗中有两个lfb1物种群；和L.borgpetersenii，只有人类一个lfb1物种群。lfb1种-群问号乳球菌SG1，对应于血清变种的翼瘤或Copenhageni，经常从人类和狗中检索（n=67/110;60.9％和n=59/60;98.3％）。尽管接种了疫苗，但仍有很大比例的受影响的狗患上了这种疾病（n=30/60;50％）。多态性lfb1基因的基因分型既健壮又简单。这种方法提供了法国大陆急性感染的钩端螺旋体菌株的第一张全球图片，基于生物样本，但不需要培养。鉴定流行的钩端螺旋体菌株及其随时间的变化将有助于对易感和水库物种进行更精确的流行病学监测。它还应促进对环境污染的监测，有可能实施预防措施并减轻这种疾病的负担。
