PDF(Pubmed)
Abstract：
Most of the functions of LC3/GABARAP in macroautophagy/autophagy are considered to depend on their association with the phagophore membrane through a conjugation to a lipid. Using site-directed mutagenesis, we inhibited the conjugation of LGG-1, the single homolog of GABARAP in C. elegans. Mutants that express only cytosolic forms revealed an essential role for the cleaved form of LGG-1 in autophagy but also in an autophagy-independent embryonic function.
摘要：
LC3/GABARAP在巨自噬/自噬中的大多数功能被认为取决于它们通过与脂质缀合而与吞噬胞膜的关联。使用定点诱变，我们抑制了线虫中GABARAP的单一同源物LGG-1的缀合。仅表达胞质形式的突变体揭示了LGG-1的切割形式在自噬中以及在自噬非依赖性胚胎功能中的重要作用。
