%0 Journal Article
%T Membrane localization of LGG-1/GABARAP is dispensable for autophagy in C. elegans.
%A Leboutet R
%A Largeau C
%A Culetto E
%A Lefebvre C
%A Hoppe T
%A Legouis R
%J Autophagy
%V 19
%N 12
%D 2023 12 23
%M 37612881
%F 13.391
%R 10.1080/15548627.2023.2249393
%X Most of the functions of LC3/GABARAP in macroautophagy/autophagy are considered to depend on their association with the phagophore membrane through a conjugation to a lipid. Using site-directed mutagenesis, we inhibited the conjugation of LGG-1, the single homolog of GABARAP in C. elegans. Mutants that express only cytosolic forms revealed an essential role for the cleaved form of LGG-1 in autophagy but also in an autophagy-independent embryonic function.