{Reference Type}: Journal Article
{Title}: Membrane localization of LGG-1/GABARAP is dispensable for autophagy in C. elegans.
{Author}: Leboutet R;Largeau C;Culetto E;Lefebvre C;Hoppe T;Legouis R;
{Journal}: Autophagy
{Volume}: 19
{Issue}: 12
{Year}: 2023 12 23
{Factor}: 13.391
{DOI}: 10.1080/15548627.2023.2249393
{Abstract}: Most of the functions of LC3/GABARAP in macroautophagy/autophagy are considered to depend on their association with the phagophore membrane through a conjugation to a lipid. Using site-directed mutagenesis, we inhibited the conjugation of LGG-1, the single homolog of GABARAP in C. elegans. Mutants that express only cytosolic forms revealed an essential role for the cleaved form of LGG-1 in autophagy but also in an autophagy-independent embryonic function.