PDF(Pubmed)
Abstract：
It is very important to generate phenotypic results that are reliable when processing chronological old skeletal remains for cases involving the identification of missing persons. To improve the success of pigmentation prediction in Second World War victims, three bones from each of the eight skeletons analyzed were included in the study, which makes it possible to generate a consensus profile. The PowerQuant System was used for quantification, the ESI 17 Fast System was used for STR typing, and a customized version of the HIrisPlex panel was used for PCR-MPS. The HID Ion Chef Instrument was used for library preparation and templating. Sequencing was performed with the Ion GeneStudio S5 System. Identical full profiles and identical hair and eye color predictions were achieved from three bones analyzed per skeleton. Blue eye color was predicted in five skeletons and brown in three skeletons. Blond hair color was predicted in one skeleton, blond to dark blond in three skeletons, brown to dark brown in two skeletons, and dark brown to black in two skeletons. The reproducibility and reliability of the results proved the multisample analysis method to be beneficial for phenotyping chronological old skeletons because differences in DNA yields in different bone types provide a greater possibility of obtaining a better-quality consensus profile.
摘要：
对于涉及识别失踪人员的情况，在处理按时间顺序排列的旧骨骼遗骸时，产生可靠的表型结果非常重要。为了提高第二次世界大战受害者的色素沉着预测的成功率，研究中包括分析的八个骨骼中的每个骨骼的三个骨骼，这使得形成共识成为可能。PowerQuant系统用于定量，ESI17快速系统用于STR分型，和定制版本的HIrisPlex面板用于PCR-MPS。HID离子厨师仪器用于文库制备和模板化。用IonGeneStudioS5系统进行测序。从每个骨骼分析的三个骨骼获得相同的完整轮廓以及相同的头发和眼睛颜色预测。在五个骨骼中预测了蓝眼睛的颜色，在三个骨骼中预测了棕色。在一个骨骼中预测到金色的头发颜色，金发到深金发三个骨架，棕色到深棕色的两个骨骼，和深棕色到黑色的两个骨架。结果的可重复性和可靠性证明，多样本分析方法对于按时间顺序对旧骨骼进行表型分析是有益的，因为不同骨骼类型中DNA产量的差异提供了更大的可能性，可以获得质量更好的共识谱。
