PDF(Pubmed)
Abstract：
Tetrodotoxin (TTX) and its analogues are naturally occurring toxins historically responsible for human poisoning fatalities in Eastern Asia. It is typically linked to the consumption of pufferfish and, to a lesser extent, marine gastropods and crabs. In the scope of a comprehensive project to understand the prevalence of emergent toxins in edible marine organisms, we report, for the first time, the detection of TTX analogues in the soft tissues of edible crabs, the European fiddler crab (Afruca tangeri) and green crab (Carcinus maenas), harvested in southern Portugal. No TTX was detected in the analyzed samples. However, three TTX analogues were detected-an unknown TTX epimer, deoxyTTX, and trideoxyTTX. These three analogues were found in the European fiddler crab while only trideoxyTTX was found in the green crab, suggesting that the accumulation of TTX analogues might be influenced by the crabs\' different feeding ecology. These results highlight the need to widely monitor TTX and its analogues in edible marine species in order to provide adequate information to the European Food Safety Authority and to protect consumers.
摘要：
河豚毒素（TTX）及其类似物是天然存在的毒素，历史上是东亚人类中毒死亡的原因。它通常与河豚的消费有关，在较小程度上，海洋腹足类和螃蟹。在一个全面项目的范围内，以了解可食用海洋生物中新兴毒素的流行情况，我们报告,第一次,TTX类似物在食用蟹的软组织中的检测，欧洲提琴蟹（Apucatangeri）和绿色蟹（Carcinusmaenas），在葡萄牙南部收获。在分析的样品中没有检测到TTX。然而,检测到三种TTX类似物-一种未知的TTX差向异构体，脱氧TTX,和三脱氧TTX。这三种类似物是在欧洲提琴蟹中发现的，而在绿蟹中只发现了三脱氧TTX，表明TTX类似物的积累可能受到螃蟹不同饲养生态的影响。这些结果强调了需要广泛监测可食用海洋物种中的TTX及其类似物，以便向欧洲食品安全局提供足够的信息并保护消费者。
