PDF(Pubmed)
Abstract：
Tetracapsuloides bryosalmonae is a malacosporean endoparasite that infects a wide range of salmonids and causes proliferative kidney disease (PKD). Brown trout serves as a carrier host whereas rainbow trout represents a dead-end host. We thus asked if the parasite adapts to the different hosts by changing molecular mechanisms. We used fluorescent activated cell sorting (FACS) to isolate parasites from the kidney of brown trout and rainbow trout following experimental infection with T. bryosalmonae. The sorted parasite cells were then subjected to RNA sequencing. By this approach, we identified 1120 parasite transcripts that were expressed differentially in parasites derived from brown trout and rainbow trout. We found elevated levels of transcripts related to cytoskeleton organisation, cell polarity, peptidyl-serine phosphorylation in parasites sorted from brown trout. In contrast, transcripts related to translation, ribonucleoprotein complex biogenesis and subunit organisation, non-membrane bounded organelle assembly, regulation of protein catabolic process and protein refolding were upregulated in rainbow trout-derived parasites. These findings show distinct molecular adaptations of parasites, which may underlie their distinct outcomes in the two hosts. Moreover, the identification of these differentially expressed transcripts may enable the identification of novel drug targets that may be exploited as treatment against T. bryosalmonae. We here also describe for the first time how FACS based isolation of T. bryosalmonae cells from infected kidney of fish fosters research and allows to define differentially expressed parasite transcripts in carrier and dead-end fish hosts.
摘要：
苔藓三叶草是一种内寄生虫，感染多种沙门氏菌并引起增殖性肾脏疾病（PKD）。棕鳟鱼充当载体宿主，而虹鳟鱼代表死胡同宿主。因此，我们询问寄生虫是否通过改变分子机制来适应不同的宿主。我们使用荧光激活细胞分选（FACS）从实验性感染T.bryosalmonae的棕鳟鱼和虹鳟鱼的肾脏中分离出寄生虫。然后对分选的寄生虫细胞进行RNA测序。通过这种方法，我们确定了1120种寄生虫转录本，它们在来自褐鳟鱼和虹鳟鱼的寄生虫中差异表达。我们发现与细胞骨架组织有关的转录水平升高，细胞极性，从鳟鱼中分选的寄生虫中的肽基丝氨酸磷酸化。相比之下,与翻译有关的成绩单，核糖核蛋白复合物的生物发生和亚基组织，非膜结合细胞器组装，在虹鳟鱼来源的寄生虫中，蛋白质分解代谢过程和蛋白质重折叠的调节被上调。这些发现显示了寄生虫的独特分子适应，这可能是他们在两个宿主中不同结果的基础。此外，这些差异表达的转录物的鉴定可以使得能够鉴定新的药物靶标,这些靶标可以用作针对苔藓沙门氏菌的治疗。我们在这里还首次描述了基于FACS的从受感染的鱼类肾脏中分离苔藓沙门氏菌细胞如何促进研究，并允许定义载体和死胡同鱼类宿主中差异表达的寄生虫转录本。
