背景:植物发育的最广泛认可的生物刺激剂之一;是低聚海藻酸盐,它调节植物的生物过程,并在园艺领域用作植物生长调节剂。当前研究的计划是研究,然而,叶面施用未辐照和辐照的海藻酸钠(UISA和ISA)以改善生长,生理活动,和埃及卷心菜植物的其他活性成分。降解的藻酸钠配备了将固态的藻酸钠暴露于不同剂量水平(0.0、25、50、75和100kGy)的伽马射线。通过FTIR对不同剂量水平的γ辐射剥夺获得的寡藻酸盐进行了表征,XRD,TGA,SEM,和TEM。不同浓度的辐照海藻酸钠,剂量水平为100kGy(200、400、600和800ppm,以及用作对照的去离子水)在田间移植卷心莴苣幼苗后,每周用手动喷雾器喷洒,直到收获阶段。对形态性状进行了评价,以及颜料,抗坏血酸,酚类物质,黄酮类化合物,可溶性蛋白质,和抗氧化活性。
结果:辐照海藻酸钠导致海藻酸钠解聚为小分子量寡糖,最佳剂量为100kGy。通过FTIR分析观察到一般结构中的某些化学修饰。在3329cm-1和1599cm-1的两个吸收带被识别为O-H和C-O拉伸,分别,并且在1411cm-1处达到的峰代表与钠离子连接的COO-拉伸基团。在1028cm-1处获得的峰是由于C-O的拉伸振动。TGA的结果提供,与UISA(43.39%)相比,ISA中的最低体重提示为100kGy(28.12%)。TEM图像指出海藻酸钠呈球状,在100kGy的ISA中,颗粒分布在12.8和21.7nm之间。辐照的海藻酸钠引起营养生长性状的显着增强(叶面积,茎长度,头部重量,和叶子编号)。通过喷涂400ppm,ISA显示总色素的最大增加(2.209mg/gFW),抗坏血酸(3.13毫克/克鲜重),酚类(1.399mg/gFW),黄酮类化合物(0.775mg/gFW),和抗氧化活性(82.14。%).此外,叶面积之间存在相关系数(R值),茎长度,头部重量,和总色素含量的叶数值,抗氧化活性,总可溶性蛋白质,和抗坏血酸。
结论:最近的调查结果表明,使用喷雾辐照的海藻酸钠(100kGy)可以改善所考虑的特性。
BACKGROUND: One of the most widely recognized biostimulators of plant development; is oligoalginate, which regulates the biological processes of plants and was used in horticultural fields as a plant growth regulator. The plan of the current research was to study, however, the foliar application of un-irradiated and irradiated Na-alginate (UISA and ISA) to improve the growth, physiological activity, and other active components of the Egyptian iceberg lettuce plant. Degraded Na-alginate is equipped with exposure of sodium alginate in its solid state to gamma-rays at different dose levels (0.0, 25, 50, 75, and 100 kGy). The characterization of the oligo-alginates achieved by γ-radiation deprivation at different dose levels was performed by FTIR, XRD, TGA, SEM, and TEM. Different concentrations of irradiated sodium alginate at dose levels of 100 kGy (200, 400, 600, and 800 ppm, as well as deionized water used as a control) were sprayed with a hand sprayer every week after transplanting the iceberg lettuce seedlings in the field until the harvest stage. Morphological traits were evaluated, as well as pigments, ascorbic acid, phenols, flavonoids, soluble proteins, and antioxidant activity.
RESULTS: Irradiated Na-alginate resulted in the depolymerization of Na-alginate into small molecular-weight oligosaccharides, and the best dose to use was 100 kGy. Certain chemical modifications in the general structure were observed by FTIR analysis. Two absorbed bands at 3329 cm-1 and 1599 cm-1, were recognized that are assigned to O-H and C-O stretching, respectively, and peaks achieved at 1411 cm-1 represent the COO-stretching group connected to the sodium ion. The peak obtained at 1028 cm-1 was owing to the stretching vibration of C-O. The results of TGA provided that the minimum weight reminder was in the ISA at 100 kGy (28.12%) compared to the UISA (43.39%). The images of TEM pointed out that the Na-alginate was globular in shape, with the particle distribution between 12.8 and 21.7 nm in ISA at 100 kGy. Irradiated sodium alginate caused a noteworthy enhancement in the vegetative growth traits (leaf area, stem length, head weight, and leaf number). By spraying 400 ppm, ISA showed a maximum increase in total pigments (2.209 mg/g FW), ascorbic acid (3.13 mg/g fresh weight), phenols (1.399 mg/g FW), flavonoids (0.775 mg/g FW), and antioxidant activities (82.14. %). Also, there were correlation coefficients (R values) between leaf area, stem length, head weight, and leaf number values with total pigment content, antioxidant activity, total soluble proteins, and ascorbic acid.
CONCLUSIONS: The outcomes of the recent investigation demonstrated that the application of spraying irradiated Na-alginate (100 kGy) resulted in an improvement of the considered characters.