Abstract：
Jujube peels have been recognized as a promising resource of several bioactive ingredients. The main composition of jujube peel polyphenols (JPP) has been identified as rutin, kaempferol-3-O-rutinosid, and salicylic acid. The JPP/zein complexes, whose bioavailability reached 69.73% ± 5.06% in vitro, have been formed successfully. The Caco-2 cell and Caenorhabditis elegans (C. elegans) models have been combined to detect the intestinal barrier protective effect of JPP and its complexes. Results showed that JPP/zein complexes contain better protection capability than JPP in both models. In the Caco-2 cell model, the complex relieved intestinal barrier damage by regulating the tight junction proteins. Moreover, the lysosome pathway has been activated, further regulating immune responses and lipid transportation, improving the barrier function of C. elegans after incubation with JPP/zein complexes according to the proteomics study. This work provides new insights into intestinal barrier protection with bioactive compounds.
摘要：
枣皮已被认为是几种生物活性成分的有希望的资源。枣皮多酚(JPP)的主要成分为芦丁,山奈酚-3-O-rutinosid,和水杨酸。JPP/玉米醇溶蛋白复合物，其体外生物利用度达到69.73%±5.06%，已经成功形成。Caco-2细胞和秀丽隐杆线虫(C.秀丽隐杆线虫）模型已结合检测JPP及其复合物的肠屏障保护作用。结果表明，JPP/玉米醇溶蛋白复合物在两种模型中都具有比JPP更好的保护能力。在Caco-2细胞模型中,复杂通过调节紧密连接蛋白减轻肠屏障损伤。此外，溶酶体途径已经被激活,进一步调节免疫反应和脂质运输，根据蛋白质组学研究，在与JPP/玉米醇溶蛋白复合物孵育后改善秀丽隐杆线虫的屏障功能。这项工作为生物活性化合物的肠道屏障保护提供了新的见解。
