Abstract：
Viscum coloratum (Kom.) Nakai is a well-known medicinal plant. However, the optimal harvest time for V. coloratum is unknown. Few studies were performed to analyze compound variation during storage and to improve post-harvest quality control. Our study aimed to comprehensively evaluate the quality of V. coloratum in different growth stages, and determine the dynamic variation of metabolites. Ultra-performance liquid chromatography tandem mass spectrometry was used to quantify 29 compounds in V. coloratum harvested in six growth periods, and the associated biosynthetic pathways were explored. The accumulation of different types of compounds were analyzed based on their synthesis pathways. Grey relational analysis was used to evaluate the quality of V. coloratum across different months. The compound variation during storage was analyzed by a high-temperature high-humidity accelerated test. The results showed that the quality of V. coloratum was the hightest in March, followed by November, and became the lowest in July. During storage, compounds in downstream steps of the biosynthesis pathway were first degraded to produce the upstream compounds and some low-molecular-weight organic acids, leading to an increase followed by a decrease in the content of some compounds, and resulted in a large gap during the degradation time course among different compounds. Due to the rapid rate and large degree of degradation, five compounds were tentatively designated as \"early warning components\" for quality control. This report provides reference for better understanding the biosynthesis and degradation of metabolites in V. coloratum and lays a theoretical foundation for rational application of V. coloratum and better quality control of V. coloratum during storage.
摘要：
彩色Viscumcoloratum(Kom.）Nakai是一种著名的药用植物。然而,V.coloratum的最佳收获时间未知。很少进行研究来分析储存过程中的化合物变化并改善收获后的质量控制。本研究旨在综合评价不同生长期色花的品质,并确定代谢物的动态变化。采用超高效液相色谱-串联质谱法对6个生长期收获的花色弧菌中29种化合物进行定量,并探索了相关的生物合成途径。根据其合成途径分析了不同类型化合物的积累。采用灰色关联分析对不同月份的色花质量进行评价。通过高温高湿加速试验分析储存过程中的化合物变化。结果表明，3月份色花质量最高，接下来是11月,并在7月达到最低。储存期间,生物合成途径的下游步骤中的化合物首先被降解以产生上游化合物和一些低分子量有机酸，导致某些化合物的含量增加，然后减少，并导致不同化合物在降解时间过程中存在很大差距。由于速度快、降解程度大,五个化合物暂时被指定为质量控制的“预警成分”。该报告为更好地理解色花中代谢产物的生物合成和降解提供了参考，为色花的合理应用和更好地控制色花在贮藏期间的质量奠定了理论基础。
