PDF(Pubmed)
Abstract：
Norovirus (NoV) is an important cause of viral acute gastroenteritis (AGE). To gain insights into the epidemiological characteristics and genetic diversity of NoV among children in Hubei, 1216 stool samples from children (≤ 5 years) obtained under AGE surveillance from January 2017 to December 2019 were analyzed. The results showed that NoV was responsible for 14.64% of AGE cases, with the highest detection rate in children aged 7-12 months (19.76%). Statistically significant differences were found between male and female infection rates (χ2 = 8.108, P = 0.004). Genetic analysis of RdRp and VP1 sequences showed that NoV GII genotypes were GII.4 Sydney [P31] (34.35%), GII.3 [P12] (25.95%), GII.2 [P16] (22.90%), GII.4 Sydney [P16] (12.98%), GII.17 [P17] (2.29%), GII.6 [P7] and GII.3 [P16] (each at 0.76%). GII.17 [P17] variants were divided into the Kawasaki323-like lineage and the Kawasaki308-like lineage. A unique recombination event was detected between strains of GII.4 Sydney 2012 and GII.4 Sydney 2016. Significantly, all GII.P16 sequences associated with GII.4/GII.2 obtained in Hubei were correlated with novel GII.2 [P16] variants that re-emerged in Germany in 2016. Antigenic site analysis of complete VP1 sequences from all GII.4 variants from Hubei identified notable variable residues of antibody epitopes. Genotyping under continuous AGE surveillance and observation of the antigenic sites of VP1 are important monitoring strategies for emerging NoV strains.
摘要：
诺如病毒(NoV)是病毒性急性胃肠炎(AGE)的重要病因。为了解湖北省儿童NoV的流行病学特征和遗传多样性，分析了2017年1月至2019年12月在AGE监测下获得的1216名儿童（≤5岁）的粪便样本。结果显示，NoV占AGE病例的14.64%，7-12个月儿童检出率最高(19.76%)。男性和女性感染率差异具有统计学意义（χ2=8.108，P=0.004）。RdRp和VP1序列的遗传分析表明，NoVGII基因型为GII.4Sydney[P31](34.35%)，GII.3[P12]（25.95%），GII.2[P16]（22.90%），GII.4悉尼[P16]（12.98％），GII.17[P17]（2.29%），GII.6[P7]和GII.3[P16]（各为0.76%）。GII.17[P17]变体分为类似Kawasaki323的谱系和类似Kawasaki308的谱系。在2012年悉尼GII.4和2016年悉尼GII.4菌株之间检测到独特的重组事件。重要的是,所有的GII。在湖北获得的与GII.4/GII.2相关的P16序列与2016年在德国重新出现的新型GII.2[P16]变体相关。来自湖北的所有GII.4变体的完整VP1序列的抗原位点分析鉴定了抗体表位的显著可变残基。在连续AGE监测下进行基因分型和观察VP1的抗原位点是新兴NoV毒株的重要监测策略。
