Abstract：
Recently, phosphatidylglycerol (PG) focused on its important role in chloroplast photosynthesis, mitochondrial function of the sperm, an inhibitory effect on SARS-CoV-2 ability to infect naïve cells, and reducing lung inflammation caused by coronavirus disease 2019. To develop an enzymatic PG determination method as the high-throughput analysis of PG, a PG-specific phospholipase C (PG-PLC) was found in the culture supernatant of Amycolatopsis sp. NT115. PG-PLC (54 kDa by SDS-PAGE) achieved the maximal activity at pH 6.0 and 55 °C and was inhibited by detergents, such as Briji35, Tween 80, and sodium cholate, but not by EDTA and metal ions, except for Zn2+. The open reading frame of the PG-PLC gene consisted of 1620 bp encoding 515-amino-acid residues containing the preceding 25-amino-acid residues (Tat signal peptide sequence). The putative amino acid sequence of PG-PLC was highly similar to those of metallophosphoesterases; however, its substrate specificity was completely different from those of known PLCs.
摘要：
最近,磷脂酰甘油（PG）在叶绿体光合作用中的重要作用，精子的线粒体功能，对SARS-CoV-2感染幼稚细胞的能力的抑制作用，并减少2019年冠状病毒病引起的肺部炎症。开发一种酶促PG测定方法作为PG的高通量分析,在扁桃体的培养上清液中发现了PG特异性磷脂酶C（PG-PLC）。NT115。PG-PLC（SDS-PAGE为54kDa）在pH6.0和55°C下达到最大活性，并受到洗涤剂的抑制，如Briji35、吐温80和胆酸钠，但不是EDTA和金属离子，除了Zn2+。PG-PLC基因的开放阅读框由1620bp组成，编码515个氨基酸残基，其中包含前面的25个氨基酸残基（Tat信号肽序列）。PG-PLC的推定氨基酸序列与金属磷酸酯酶的氨基酸序列高度相似；然而，其底物特异性与已知PLC完全不同.
