异黄酮,由于对人类和植物健康都有好处,因此感兴趣的主要次生代谢物,完全由豆类生产。在这项研究中,我们对211大豆[Glycinemax(L.)合并。]在五个环境中增长的加入。在种质之间观察到广泛而明显的表型差异,regions,和多年的成长。与糖素(GLE)总和相关的二十六个单核苷酸多态性(SNP),缩水甘油(GL),6″-O-乙酰缩水甘油(AGL),通过全基因组关联研究(GWAS)在多种环境中检测到6″-O-丙二酰缩水甘油(MGL)含量。这些SNP位于11号染色体上(8,148,438bp至8,296,956bp,更名为qGly11-01)。Glyma.11g108300(GmGLY1),编码P450家族蛋白的基因,通过序列变异分析鉴定,功能注释,加权基因共表达网络分析(WGCNA),和候选基因的表达谱分析,大豆毛状根的转化。GmGLY1的过表达增加了大豆毛状根和转基因种子中的糖素含量(GLC),而CRISPR/Cas9产生的突变体表现出降低的GLC和增加的大豆苷元含量(DAC)。单倍型分析表明,GmGLY1等位基因变异显着影响GLC的积累。这些发现增强了我们对影响大豆GLC的基因的理解,并可能指导具有高且稳定GLC的品系的育种。
Isoflavones, the major secondary metabolites of interest due to their benefits to both human and plant health, are exclusively produced by legumes. In this study, we profiled the isoflavone content in dry seeds from 211 soybean [Glycine max (L.) Merr.] accessions grown across five environments. Broad and discernible phenotypic variations were observed among accessions, regions, and years of growth. Twenty-six single-nucleotide polymorphisms (SNPs) associated with the sum of glycitein (GLE), glycitin (GL), 6″-O-acetylglycitin (AGL), and 6″-O-malonylglycitin (MGL) contents were detected in multiple environments via a genome-wide association study (GWAS). These SNPs were located on chromosome 11 (8,148,438 bp to 8,296,956 bp, renamed qGly11-01). Glyma.11g108300 (GmGLY1), a gene that encodes a P450 family protein, was identified via sequence variation analysis, functional annotation, weighted gene coexpression network analysis (WGCNA), and expression profile analysis of candidate gene, and hairy roots transformation in soybean. Overexpression of GmGLY1 increased the glycitein content (GLC) in soybean hairy roots and transgenic seeds, while CRISPR/Cas9-generated mutants exhibited decreased GLC and increased daidzein content (DAC). Haplotype analysis revealed that GmGLY1 allelic variations significantly affect the GLC accumulation. These findings enhance our understanding of genes influencing GLC in soybean and may guide breeding for lines with high and stable GLC.