一种新的内生放线菌,菌株MEP2-6T,从MaeFagMai分区收集的马铃薯块茎的结痂组织中分离出来,SanSaiDistrict,清迈省,泰国。菌株MEP2-6T是革兰氏阳性丝状细菌,其特征是细胞壁肽聚糖和阿拉伯糖中的内消旋二氨基庚二酸,半乳糖,葡萄糖,和全细胞水解产物中的核糖。二磷脂酰甘油,磷脂酰甘油,磷脂酰乙醇胺,和羟基磷脂酰乙醇胺是主要的磷脂,其中MK-9(H6)是主要的甲基萘醌,而iso-C16:0和iso-C15:0是主要的细胞脂肪酸。该菌株的基因组大小为10,277,369bp,GC含量为71.7%。16SrRNA基因系统发育和核心系统发育分析显示,菌株MEP2-6T与丝豆科NRRLB-24131T密切相关(99.4%),A.pretoriensisDSM44654T(99.3%),和A.eburneaGLM-1T(98.9%)。值得注意的是,菌株MEP2-6T显示91.7%,91.8%,87%的ANIb和49%,48.8%,和35.4%dDDH到A.LexingtonensisDSM44653T(=NRRLB-24131T),A.eburneaGLM-1T,和A.pretoriensisDSM44654T,分别。基于表型,化学分类学,和基因组数据,菌株MEP2-6T可以被正式分配给霉菌属中的一个新物种,为此命名为solaniAmycolatopsissp。11月。已被提议。应变类型为MEP2-6T(=JCM36309T=TBRC17632T=NBRC116395T)。由于未观察到幼苗发育迟缓和马铃薯块茎切片上的坏死病变,因此强烈证明了枯萎病MEP2-6T是马铃薯sc病的非植物病原体,并且没有与植物毒素诱导的结痂病变的BGC相关的核心生物合成基因。此外,比较基因组学可以更好地理解使A.solaniMEP2-6T适应植物内圈的遗传机制。重要的是,菌株smBGC容纳了由几种生物活性化合物编码的33个smBGC,可以有益地应用于农业和医药领域。因此,菌株MEP2-6T是一种有希望的候选作为一种新型的生物防治剂和抗生素生产者。
A novel endophytic actinomycete, strain MEP2-6T, was isolated from scab tissues of potato tubers collected from Mae Fag Mai Sub-district, San Sai District, Chiang Mai Province, Thailand. Strain MEP2-6T is a gram-positive filamentous bacteria characterized by meso-diaminopimelic acid in cell wall peptidoglycan and arabinose, galactose, glucose, and ribose in whole-cell hydrolysates. Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and hydroxy-phosphatidylethanolamine were the major phospholipids, of which MK-9(H6) was the predominant menaquinone, whereas iso-C16:0 and iso-C15:0 were the major cellular fatty acids. The genome of the strain was 10,277,369 bp in size with a G + C content of 71.7%. The 16S rRNA gene phylogenetic and core phylogenomic analyses revealed that strain MEP2-6T was closely related to
Amycolatopsis lexingtonensis NRRL B-24131T (99.4%), A. pretoriensis DSM 44654T (99.3%), and A. eburnea GLM-1T (98.9%). Notably, strain MEP2-6T displayed 91.7%, 91.8%, and 87% ANIb and 49%, 48.8%, and 35.4% dDDH to A. lexingtonensis DSM 44653T (=NRRL B-24131T), A. eburnea GLM-1T, and A. pretoriensis DSM 44654T, respectively. Based on phenotypic, chemotaxonomic, and genomic data, strain MEP2-6T could be officially assigned to a novel species within the genus
Amycolatopsis, for which the name
Amycolatopsis solani sp. nov. has been proposed. The type of strain is MEP2-6T (=JCM 36309T = TBRC 17632T = NBRC 116395T).
Amycolatopsis solani MEP2-6T was strongly proven to be a non-phytopathogen of potato scab disease because stunting of seedlings and necrotic lesions on potato tuber slices were not observed, and there were no core biosynthetic genes associated with the BGCs of phytotoxin-inducing scab lesions. Furthermore, comparative genomics can provide a better understanding of the genetic mechanisms that enable A. solani MEP2-6T to adapt to the plant endosphere. Importantly, the strain smBGCs accommodated 33 smBGCs encoded for several bioactive compounds, which could be beneficially applied in the fields of agriculture and medicine. Consequently, strain MEP2-6T is a promising candidate as a novel biocontrol agent and antibiotic producer.