Abstract：
In this study, three strains of heterotrophic nitrification-aerobic denitrification (HN-AD) capable of simultaneously removing phosphorus were isolated from activated sludge, and low-temperature coconut shell biochar was prepared. The metabolic effects of combined HN-AD bacteria on the total nitrogen (TN) and total phosphorus (TP) were investigated, and the enhanced efficiency and mechanism of low-temperature biochar on the combined bacteria were also explored. The results indicated that the combined bacteria could adapt to environmental impacts and multiple nitrogen sources. The low-temperature biochar containing more aliphatic carbon and oxygen-containing functional groups enhanced the metabolic activity of combined HN-AD bacteria and accelerated the electron transfer process during nitrogen and phosphorus degradation. The removal efficiencies of TN and TP increased by 68% and 88%, respectively, in the treatment of actual sewage by biochar attached with combined bacteria. The findings form a basis for the engineering utilization of HN-AD and are of great practical significance.
摘要：
在这项研究中,从活性污泥中分离出三株能同时除磷的异养硝化-好氧反硝化(HN-AD),制备了低温椰壳生物炭。研究了HN-AD复合菌对总氮(TN)和总磷(TP)的代谢影响。并探讨了低温生物炭对复合菌的增强效率和机理。结果表明，复合细菌可以适应环境影响和多种氮源。含有更多脂肪族碳和含氧官能团的低温生物炭增强了结合的HN-AD细菌的代谢活性，并加速了氮和磷降解过程中的电子转移过程。TN和TP的去除效率分别提高了68%和88%,分别,在实际污水的处理中，生物炭附着有复合细菌。研究结果为HN-AD的工程应用奠定了基础，具有重要的现实意义。
