{Reference Type}: Journal Article {Title}: Enhanced efficiency and mechanism of low-temperature biochar on simultaneous removal of nitrogen and phosphorus by combined heterotrophic nitrification-aerobic denitrification bacteria. {Author}: Jiang G;Liu Y;Liu X;Shen Y;Zhang A;Li Z; {Journal}: Bioresour Technol {Volume}: 373 {Issue}: 0 {Year}: Apr 2023 {Factor}: 11.889 {DOI}: 10.1016/j.biortech.2023.128720 {Abstract}: In this study, three strains of heterotrophic nitrification-aerobic denitrification (HN-AD) capable of simultaneously removing phosphorus were isolated from activated sludge, and low-temperature coconut shell biochar was prepared. The metabolic effects of combined HN-AD bacteria on the total nitrogen (TN) and total phosphorus (TP) were investigated, and the enhanced efficiency and mechanism of low-temperature biochar on the combined bacteria were also explored. The results indicated that the combined bacteria could adapt to environmental impacts and multiple nitrogen sources. The low-temperature biochar containing more aliphatic carbon and oxygen-containing functional groups enhanced the metabolic activity of combined HN-AD bacteria and accelerated the electron transfer process during nitrogen and phosphorus degradation. The removal efficiencies of TN and TP increased by 68% and 88%, respectively, in the treatment of actual sewage by biochar attached with combined bacteria. The findings form a basis for the engineering utilization of HN-AD and are of great practical significance.