Abstract：
Rapid and accurate detection technologies are crucial for disease prevention and control. In particular, the COVID-19 pandemic has posed a great threat to our society, highlighting the importance of rapid and highly sensitive detection techniques. In recent years, CRISPR/Cas-based gene editing technique has brought revolutionary advances in biotechnology. Due to its fast, accurate, sensitive, and cost-effective characteristics, the CRISPR-based nucleic acid detection technology is revolutionizing molecular diagnosis. CRISPR-based diagnostics has been applied in many fields, such as detection of infectious diseases, genetic diseases, cancer mutation, and food safety. This review summarized the advances in CRISPR-based nucleic acid detection systems and its applications. Perspectives on intelligent diagnostics with CRISPR-based nucleic acid detection and artificial intelligence were also provided.
摘要：
快速准确的检测技术对于疾病的预防和控制至关重要。特别是,COVID-19大流行对我们的社会构成了巨大威胁，强调快速和高灵敏度检测技术的重要性。近年来,基于CRISPR/Cas的基因编辑技术为生物技术带来了革命性的进步。由于速度快,准确,敏感,和成本效益的特点，基于CRISPR的核酸检测技术正在彻底改变分子诊断。基于CRISPR的诊断已经在许多领域得到了应用,如传染病的检测，遗传性疾病,癌症突变,和食品安全。本文综述了基于CRISPR的核酸检测系统及其应用的研究进展。还提供了关于基于CRISPR的核酸检测和人工智能的智能诊断的观点。
