Abstract：
UNASSIGNED: Methicillin-resistant Staphylococcus (MRS) has originated, spread extensively, and become a prominent source of bacterial infections in both human and animal.
UNASSIGNED: We report the prevalence, genetic diversity, and antimicrobial resistance pattern of Staphylococcus pseudintermedius and Staphylococcus aureus strains isolated from dogs and cats with eye discharges.
UNASSIGNED: A total of 12 (6.0%) coagulase-positives staphylococci were identified as (6/200, 3%) S. aureus and (6/200, 3%) S. pseudintermedius. The phenotypic methicillin resistance of S. aureus and S. pseudintermedius were 50.0% (3/6) and 16.7% (1/6), respectively. None of the isolates showed biofilm formation in the microtiter plate assay. The highest resistance (50.0%) for S. pseudintermedius strains was detected against clindamycin and tetracycline. 67.0% of S. aureus isolates were resistant to penicillin-G. The PCR analysis conducted for detection of mecA gene indicated that only one S. aureus isolated from a cat was mecA gene positive. Phylogenetic analysis based on repetitive sequence-based PCR (rep-PCR) showed that all strains were typable and generated PCR products ranging from 800 bp to 4,400 bp. The lineages ST241 and the novel ST2361 in multi-locus sequence typing (MLST) analysis were detected in one methicillin-susceptible S. pseudintermedius and methicillin-resistant S. pseudintermedius of dogs, respectively. In addition, the lineages ST4155 and ST7217 of two methicillin-resistant S. aureus strains of cats were connected epidemiologically to previously reported cases.
UNASSIGNED: These results indicate epidemiologically related strains (ST241, ST4155, and ST7217) transferring between animals and humans. Therefore, the strategies to combat the widespread MRS should be based on collaboration between human and veterinary medicine under the One Health concept.
UNASSIGNED: We report the prevalence, genetic diversity, and antimicrobial resistance pattern of Staphylococcus pseudintermedius and Staphylococcus aureus strains isolated from dogs and cats with eye discharges.
UNASSIGNED: A total of 12 (6.0%) coagulase-positives staphylococci were identified as (6/200, 3%) S. aureus and (6/200, 3%) S. pseudintermedius. The phenotypic methicillin resistance of S. aureus and S. pseudintermedius were 50.0% (3/6) and 16.7% (1/6), respectively. None of the isolates showed biofilm formation in the microtiter plate assay. The highest resistance (50.0%) for S. pseudintermedius strains was detected against clindamycin and tetracycline. 67.0% of S. aureus isolates were resistant to penicillin-G. The PCR analysis conducted for detection of mecA gene indicated that only one S. aureus isolated from a cat was mecA gene positive. Phylogenetic analysis based on repetitive sequence-based PCR (rep-PCR) showed that all strains were typable and generated PCR products ranging from 800 bp to 4,400 bp. The lineages ST241 and the novel ST2361 in multi-locus sequence typing (MLST) analysis were detected in one methicillin-susceptible S. pseudintermedius and methicillin-resistant S. pseudintermedius of dogs, respectively. In addition, the lineages ST4155 and ST7217 of two methicillin-resistant S. aureus strains of cats were connected epidemiologically to previously reported cases.
UNASSIGNED: These results indicate epidemiologically related strains (ST241, ST4155, and ST7217) transferring between animals and humans. Therefore, the strategies to combat the widespread MRS should be based on collaboration between human and veterinary medicine under the One Health concept.
摘要：
未经批准：耐甲氧西林葡萄球菌（MRS）起源于，广泛传播，并成为人类和动物细菌感染的主要来源。
未经评估：我们报告了患病率，遗传多样性,以及从有眼部分泌物的狗和猫中分离出的假中介葡萄球菌和金黄色葡萄球菌菌株的耐药性模式。
未经证实：总共12例(6.0%)凝固酶阳性葡萄球菌被鉴定为(6/200,3%)金黄色葡萄球菌和(6/200,3%)假中介葡萄球菌。金黄色葡萄球菌和假中介葡萄球菌的甲氧西林耐药率分别为50.0%(3/6)和16.7%(1/6),分别。在微量滴定板测定中没有分离物显示生物膜形成。对克林霉素和四环素的耐药性最高（50.0％）。67.0%的金黄色葡萄球菌对青霉素G耐药。为了检测mecA基因而进行的PCR分析显示,仅从猫分离的一个金黄色葡萄球菌是mecA基因阳性的。基于重复序列的PCR（rep-PCR）的系统发育分析表明，所有菌株均可分型，并产生800bp至4,400bp的PCR产物。在一种对甲氧西林敏感的假中间杆菌和耐甲氧西林的假中间杆菌中检测到了多基因座序列分型（MLST）分析中的谱系ST241和新型ST2361。分别。此外,两种耐甲氧西林金黄色葡萄球菌猫菌株的谱系ST4155和ST7217在流行病学上与以前报告的病例相关.
UNASSIGNED：这些结果表明流行病学相关的菌株（ST241，ST4155和ST7217）在动物和人类之间转移。因此,应对广泛的MRS的策略应基于人类和兽医学在“一个健康”概念下的合作。
未经评估：我们报告了患病率，遗传多样性,以及从有眼部分泌物的狗和猫中分离出的假中介葡萄球菌和金黄色葡萄球菌菌株的耐药性模式。
未经证实：总共12例(6.0%)凝固酶阳性葡萄球菌被鉴定为(6/200,3%)金黄色葡萄球菌和(6/200,3%)假中介葡萄球菌。金黄色葡萄球菌和假中介葡萄球菌的甲氧西林耐药率分别为50.0%(3/6)和16.7%(1/6),分别。在微量滴定板测定中没有分离物显示生物膜形成。对克林霉素和四环素的耐药性最高（50.0％）。67.0%的金黄色葡萄球菌对青霉素G耐药。为了检测mecA基因而进行的PCR分析显示,仅从猫分离的一个金黄色葡萄球菌是mecA基因阳性的。基于重复序列的PCR（rep-PCR）的系统发育分析表明，所有菌株均可分型，并产生800bp至4,400bp的PCR产物。在一种对甲氧西林敏感的假中间杆菌和耐甲氧西林的假中间杆菌中检测到了多基因座序列分型（MLST）分析中的谱系ST241和新型ST2361。分别。此外,两种耐甲氧西林金黄色葡萄球菌猫菌株的谱系ST4155和ST7217在流行病学上与以前报告的病例相关.
UNASSIGNED：这些结果表明流行病学相关的菌株（ST241，ST4155和ST7217）在动物和人类之间转移。因此,应对广泛的MRS的策略应基于人类和兽医学在“一个健康”概念下的合作。
