Abstract：
The technology of long reads substantially improved the contingency of the genome assembly, particularly resolving contiguity of the repetitive regions. By integrating the interactive fragment using Hi-C, and the HiFi technique, a solid genome of the honeybee Apis mellifera carnica was assembled at the chromosomal level. A distinctive pattern of genes involved in social evolution was found by comparing it with social and solitary bees. A positive selection was identified in genes involved with cold tolerance, which likely underlies the adaptation of this European honeybee subspecies in the north hemisphere. The availability of this new high-quality genome will foster further studies and advances on genome variation during subspeciation, honeybee breeding and comparative genomics.
摘要：
长读技术大大改善了基因组组装的偶然性，特别是解决重复性区域的连续性。通过使用Hi-C集成交互式片段，和HiFi技术，在染色体水平上组装了蜜蜂的固体基因组。通过将其与社交和独居蜜蜂进行比较，发现了参与社会进化的独特基因模式。在与耐寒性有关的基因中鉴定出阳性选择，这可能是北半球这种欧洲蜜蜂亚种适应的基础。这种新的高质量基因组的可用性将促进亚种期间基因组变异的进一步研究和进步，蜜蜂育种和比较基因组学。
