Abstract：
Initially, natural antisense transcripts (NATs, natRNAs, or asRNAs) were considered repressors; however, their functions in gene regulation are diverse. Positive, negative, or neutral correlations to the cognate gene expression have been noted. Although the first studies were published about 50 years ago, there is still much to be investigated regarding antisense transcripts in plants. A systematic review of scientific publications available in the Web of Science databases was conducted to contextualize how the studying of antisense transcripts has been addressed. Studies were classified considering three categories: \"Natural antisense\" (208), artificial antisense used in \"Genetic Engineering\" (797), or \"Natural antisense and Genetic Engineering\"-related publications (96). A similar string was used for a systematic search in the NCBI Gene database. Of the 1132 antisense sequences found for plants, only 0.8% were cited in PubMed and had antisense information confirmed. This value was the lowest when compared to fungi (2.9%), bacteria (2.3%), and mice (54.1%). Finally, we present an update for the cis-NATs identified in Saccharum spp. Of the 1413 antisense transcripts found in different experiments, 25 showed concordant expressions, 22 were discordant, 1264 did not correlate with the cognate genes, and 102 presented variable results depending on the experiment.
摘要：
最初，天然反义转录本(NAT，natRNAs,或asRNA)被认为是阻遏物；然而，它们在基因调控中的功能是多种多样的。阳性,负,或与同源基因表达的中性相关性已被注意到。尽管第一批研究发表于大约50年前，关于植物中的反义转录物仍有许多研究。对WebofScience数据库中提供的科学出版物进行了系统审查，以了解如何解决反义转录本的研究。研究分为三类：“天然反义”(208)，人工反义用于“基因工程”(797)，或“天然反义和基因工程”相关出版物(96)。在NCBI基因数据库中使用类似的字符串进行系统搜索。在为植物发现的1132个反义序列中，只有0.8%在PubMed中被引用，并且反义信息得到证实。与真菌（2.9％）相比，该值最低，细菌(2.3%)，和小鼠（54.1%）。最后,我们提供了在糖果类中鉴定的顺式NAT的更新。在不同实验中发现的1413个反义转录本，25显示一致的表达，22不和谐，1264与同源基因无关，和102呈现了取决于实验的变量结果。
