Abstract：
Urinary tract infections represent a world public health problem, which is caused mainly by Uropathogenic Escherichia coli. Although they are originally found in the intestinal microbiota in the majority of the cases, urinary tract infections can also be caused by intra-intestinal pathogenic E. coli.
The main objective of our research is to identify the virulence factors generally associated with different pathotypes across phylogenetic groups.
E. coli were isolated from patients with urinary tract infections. Antimicrobial susceptibility tests, virulence genes and phylogroups were prospected. The data analysis were performed using the chi-square and Fisher exact test.
In total, 72.2% of isolates showed multidrug resistant. We have also depicted an important association between E. coli from inpatients with UTIs and pap and hlyA genes (p-0.041 and p-0.019 respectively). The predominant phylogenetic group in our isolates is B2 (45.4%) followed by D (12.4%). Our results showed that 9.3% of isolates have an unknown phylogroup which shows a significant association with astA gene (p-0.008). We have as well found a significant association between B2 and three virulence genes namely pap, hlyA and invE (p-0.002, p-0.001, p-0.025 respectively); B1 and pap, hlyA genes (p-0.049 and p-0.021 respectively); E and afa gene (p-0.024).
Certain virulence factors have been shown to be potential targets for drug design and therapeutic pathways in order to deal with the antimicrobial resistance problem enhanced by antibiotic therapy.
The main objective of our research is to identify the virulence factors generally associated with different pathotypes across phylogenetic groups.
E. coli were isolated from patients with urinary tract infections. Antimicrobial susceptibility tests, virulence genes and phylogroups were prospected. The data analysis were performed using the chi-square and Fisher exact test.
In total, 72.2% of isolates showed multidrug resistant. We have also depicted an important association between E. coli from inpatients with UTIs and pap and hlyA genes (p-0.041 and p-0.019 respectively). The predominant phylogenetic group in our isolates is B2 (45.4%) followed by D (12.4%). Our results showed that 9.3% of isolates have an unknown phylogroup which shows a significant association with astA gene (p-0.008). We have as well found a significant association between B2 and three virulence genes namely pap, hlyA and invE (p-0.002, p-0.001, p-0.025 respectively); B1 and pap, hlyA genes (p-0.049 and p-0.021 respectively); E and afa gene (p-0.024).
Certain virulence factors have been shown to be potential targets for drug design and therapeutic pathways in order to deal with the antimicrobial resistance problem enhanced by antibiotic therapy.
摘要：
背景：尿路感染是世界公共卫生问题，这主要是由尿路致病性大肠杆菌引起的。虽然,在大多数情况下，它们最初存在于肠道微生物群中，尿路感染也可由肠道内致病性大肠杆菌引起。
目的：我们研究的主要目的是确定通常与系统发育群体中不同病理类型相关的毒力因子。
方法：E.从尿路感染患者中分离出大肠杆菌。抗菌药物敏感性试验,对毒力基因和系统群进行了展望。使用卡方和Fisher精确检验进行数据分析。
结果：总计，72.2%的分离株出现多重耐药。我们还描述了来自患有UTI的住院患者的大肠杆菌与pap和hlyA基因之间的重要关联(分别为p-0.041和p-0.019)。在我们的分离物中，主要的系统发育组是B2（45.4％），其次是D（12.4％）。我们的结果表明，9.3％的分离株具有未知的系统群，其与astA基因显着相关（p-0.008）。我们也发现了B2和三个毒力基因之间的显著关联，即pap，hlyA和invE（分别为p-0.002，p-0.001，p-0.025）；B1和pap，hlyA基因(分别为p-0.049和p-0.021);E和afa基因(p-0.024)。
结论：某些毒力因子已被证明是药物设计和治疗途径的潜在靶标，以应对抗生素治疗增强的抗菌素耐药性问题。
目的：我们研究的主要目的是确定通常与系统发育群体中不同病理类型相关的毒力因子。
方法：E.从尿路感染患者中分离出大肠杆菌。抗菌药物敏感性试验,对毒力基因和系统群进行了展望。使用卡方和Fisher精确检验进行数据分析。
结果：总计，72.2%的分离株出现多重耐药。我们还描述了来自患有UTI的住院患者的大肠杆菌与pap和hlyA基因之间的重要关联(分别为p-0.041和p-0.019)。在我们的分离物中，主要的系统发育组是B2（45.4％），其次是D（12.4％）。我们的结果表明，9.3％的分离株具有未知的系统群，其与astA基因显着相关（p-0.008）。我们也发现了B2和三个毒力基因之间的显著关联，即pap，hlyA和invE（分别为p-0.002，p-0.001，p-0.025）；B1和pap，hlyA基因(分别为p-0.049和p-0.021);E和afa基因(p-0.024)。
结论：某些毒力因子已被证明是药物设计和治疗途径的潜在靶标，以应对抗生素治疗增强的抗菌素耐药性问题。
