Abstract：
While a large body of literature has shown the health problems of illicit drug use, research is needed on how substance abuse impacts DNA damage and contaminants in blood, especially given Pb-contaminated opium. This pilot study aimed to evaluate the levels of lead (Pb), 8-hydroxy di-guanine (8-oxo-Gua), and malondialdehyde (MDA) in the blood serum of opium addicts and non-addict people. The current study is a case-control study with a cross-sectional design. A sample of 50 opium-addicted and non-addict adults were chosen for this study using convenience and random sampling methods. Participants were divided into two groups: addicts and non-addicts. The atomic absorption spectroscopy method was used to measure the quantity of Pb, and the Enzyme-Linked Immunosorbent Assay (ELISA) method was used to measure the amount of 8-oxo-Gua and MDA. The data were analyzed using an independent t-test. The results show that the amount of Pb in the blood serum of addicted women and men was higher than levels in non-addict men and women, for the study participants (p-value = 0.001). Blood levels were not significantly different between addicts and non-addicts for men or women for 8-oxo-Gua (p-value = 0.647 for women and p-value = 0.785 for men) and MDA (p-value = 0.867 for women and p-value = 0.995 for men). In general, addicts\' blood Pb levels were found to be substantially higher than those of normal non-addict persons in this pilot study. As a result, testing for blood Pb levels in addicts may be informative in instances when symptoms are inconclusive.
摘要：
虽然大量文献显示了非法药物使用的健康问题，需要研究药物滥用如何影响血液中的DNA损伤和污染物，尤其是受铅污染的鸦片。这项试点研究旨在评估铅（Pb）的水平，8-羟基二鸟嘌呤（8-氧代-瓜），鸦片成瘾者和非成瘾者血清中的丙二醛（MDA）。本研究是横断面设计的病例对照研究。本研究采用方便和随机抽样的方法，选取了50名鸦片成瘾者和非成瘾者的样本。参与者分为两组：成瘾者和非成瘾者。采用原子吸收光谱法测定Pb的含量,采用酶联免疫吸附试验(ELISA)方法测定8-氧代-瓜和MDA的含量。使用独立t检验分析数据。结果表明,成瘾者男女血清中铅含量均高于非成瘾者男女,对于研究参与者（p值=0.001）。对于8-oxo-Gua（女性p值=0.647，男性p值=0.785）和MDA（女性p值=0.867，男性p值=0.995），男性和非成瘾者的血液水平没有显着差异。总的来说,在这项初步研究中，发现成瘾者的血液铅水平大大高于正常的非成瘾者。因此,在症状尚无定论的情况下，对成瘾者的血铅水平进行检测可能会提供信息。
