背景:每年有数百万人被诊断患有癌症,与之抗争给社区和政府带来沉重的财政负担。在癌症领域已经取得了许多进展;最新的方法之一是使用溶瘤病毒。本研究旨在评价溶瘤新城疫病毒野生型株(NDV-WTS)对免疫系统的影响。
方法:将40只小鼠分成4组(每组10只动物)。对照组接受磷酸盐缓冲盐水,和实验组1(NDV-WTS1),实验组2(NDV-WTS2),和实验组3(NDV-WTS3)在0、14日接受了10-1、10-2和10-3滴度的新城疫病毒,第28天。第31天,将100μL新城疫病毒注射到动物的左脚垫中。48小时后,测量迟发型超敏反应(DTH).在第33天,分离腹膜巨噬细胞。然后通过甲基-噻唑基-四唑(MTT)测试测量细胞的增殖。还评估了腹膜巨噬细胞的中性红摄取和呼吸爆发。数据采用SPSS统计软件,版本19.
结果:DTH测试结果表明,对照足垫肿胀,NDV-WTS1、NDV-WTS2和NDV-WTS3组为23.5%,23.5%,23.6%和23.6%。在这方面,两组之间没有显著差异(P>0.05)。作为巨噬细胞呼吸爆发指标的硝基蓝四唑(NBT)减少试验阴性,组间无显著性差异(P>0.05)。中性红摄取试验和MTT试验显示各组间无显著差异(P>0.05)。
结论:这项研究的结果表明,10-1、10-2和10-3剂量的NDV-WTS对健康的正常细胞没有不良影响。
BACKGROUND: Millions of people are diagnosed with cancer each year, and fighting it puts a heavy financial burden on communities and governments. Numerous advances have been made in the field of cancer; one of the newest methods is using oncolytic viruses. This study aimed to evaluate the effect of oncolytic Newcastle disease virus wild-type strains (NDV-WTS) on the immune system.
METHODS: Forty mice were divided into four groups (10 animals in each group). The control group received phosphate buffered saline, and experimental group 1 (NDV-WTS 1), experimental group 2 (NDV-WTS 2), and experimental group 3 (NDV-WTS 3) received 10-1, 10-2, and 10-3 titers of Newcastle virus on 0, 14th, and 28th days. On the 31st day, 100 µL of Newcastle virus was injected into the left footpads of animals. After 48 hours, delayed-type hypersensitivity (DTH) reactions were measured. On the 33rd day, peritoneal macrophages were isolated. Then proliferation of the cells was measured by the methyl-thiazolyl-tetrazolium (MTT) test. Neutral red uptake and respiratory burst of peritoneal macrophages were also assessed. Data were analyzed using statistical software SPSS, version 19.
RESULTS: The results of the DTH test showed that footpad swelling in control, NDV-WTS 1, NDV-WTS 2, and NDV-WTS 3 groups were 23.5%, 23.5%, 23.6% and 23.6%. No significant differences were seen between the groups in this regard (P > 0.05). A negative nitroblue tetrazolium (NBT) reduction test as an indicator of macrophage\'s respiratory burst, showed no significant difference between the groups (P > 0.05). The neutral red uptake assay and MTT test showed no significant differences between the groups (P > 0.05).
CONCLUSIONS: The results of this study showed that NDV-WTS in doses of 10-1, 10-2, and 10-3 have no adverse effects on healthy normal cells.