关键词: Adenovirus prevalence serotypes viral conjunctivitis

Mesh : Humans Egypt / epidemiology Keratoconjunctivitis / diagnosis epidemiology Conjunctivitis, Viral / diagnosis epidemiology Conjunctiva Conjunctivitis Adenoviruses, Human / genetics DNA, Viral / genetics analysis

来  源:   DOI:10.1080/09273948.2022.2092004

Abstract:
UNASSIGNED: To evaluate the frequency of Human adenovirus (HAdV) and its serotypes in keratoconjunctivitis patients who attended the outpatient clinics of Mansoura Ophthalmic Center, Egypt.
UNASSIGNED: Conjunctival secretions and corneal scrapings were collected from patients complaining of clinically diagnosed viral keratoconjunctivitis. The molecular method for HAdV detection was performed by polymerase chain reaction (PCR) followed by restriction enzymes (REA) determination of serotypes for hexone gene.
UNASSIGNED: HAdV infection was detected in 38% of samples. There were 4 serotypes of Human adenovirus species D (HAdV-D) isolated (4, 8, 37, 3), where HAdV-D8 was the most dominant. Contact with infected patient, follicular conjunctivitis and subepithelial corneal infiltrates are useful features for clinical diagnosis of adenoviral conjunctivitis.
UNASSIGNED: HAdV was significant etiological factor of acute follicular conjunctivitis. Accurate diagnosis of adenoviral conjunctivitis is essential for appropriate management, reducing permanent visual impairment and to limit the transmission of the virus within the community.
摘要:
为了评估在Mansoura眼科中心门诊就诊的角膜结膜炎患者中人类腺病毒(HAdV)的频率及其血清型,埃及。
结膜分泌物和角膜刮片取自临床诊断为病毒性角膜结膜炎的患者。HAdV检测的分子方法是通过聚合酶链反应(PCR)进行的,然后是限制酶(REA)测定己酮基因的血清型。
在38%的样品中检测到HAdV感染。有4种血清型的人腺病毒物种D(HAdV-D)分离(4,8,37,3),其中HAdV-D8是最主要的。接触受感染的病人,滤泡性结膜炎和上皮下角膜浸润是临床诊断腺病毒性结膜炎的有用特征。
HAdV是急性滤泡性结膜炎的重要病因。腺病毒结膜炎的准确诊断对于适当的治疗至关重要。减少永久性视力障碍,并限制病毒在社区内的传播。
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