Abstract：
It is important to inhibit oxidative stress to maintain sperm motility during cryopreservation. The present study was performed to investigate the effects of supplementing oligomeric proanthocyanidins (OPC) and bamboo leaf flavonoids (BLF) or their combination as an extender for Simmental bull semen freezing. OPC, BLF, or their combination were added to the frozen diluent of bovine semen. Afterwards, computer-assisted semen analysis (CASA), detection of membrane functionality, acrosome integrity, mitochondrial integrity, CAT, SOD, GSH-PX, MDA, and ROS were conducted. The results showed that adding 50 mg/L OPC or 4 mg/L BLF could improve the quality of frozen sperm. Compared with 50 mg/L OPC alone, the combination of 50mg/L OPC and 2 mg/L BLF significantly increased the kinematic parameters of sperm, and sperm CAT, GSH-PX and SOD levels (p < 0.05), whereas the MDA of sperm was decreased (p < 0.05). These results indicated that compared to the addition of 50 mg/L OPC alone, a combination of 50 mg/L OPC and 2 mg/L BLF could further improve the quality of frozen semen. The results could provide theoretical data support for the development of a new protective agent and are significant for the cryopreservation of bovine semen in the future.
摘要：
在冷冻保存过程中，抑制氧化应激对维持精子运动很重要。进行本研究是为了研究补充低聚原花色素（OPC）和竹叶黄酮（BLF）或其组合作为西门塔尔公牛精液冷冻剂的作用。OPC,BLF,或它们的组合被添加到牛精液的冷冻稀释剂中。之后,计算机辅助精液分析（CASA），检测膜的功能，顶体完整性,线粒体完整性，CAT,SOD,GSH-PX,MDA,并进行了ROS。结果表明，添加50mg/LOPC或4mg/LBLF可以提高冷冻精子的质量。与单独使用50mg/LOPC相比，50mg/LOPC和2mg/LBLF的组合显着增加了精子的运动学参数，和精子CAT，GSH-PX和SOD水平（p<0.05），精子MDA降低(p<0.05)。这些结果表明，与单独添加50mg/LOPC相比，50mg/LOPC和2mg/LBLF的组合可以进一步提高冷冻精液的质量。研究结果可为新型保护剂的开发提供理论数据支持,对今后牛精液的冷冻保存具有重要意义。
