■精子产生能力受年龄的影响,这与精子形态异常有关,会影响生育能力。这项研究的目的是评估西门塔尔公牛的年龄,睾丸激素和脂联素浓度与精子异常之间的关系。
■这项研究使用了11头公牛,分成三组。第一组由五头4-5岁的公牛组成,第二和第三组各有三只公牛,6-7岁和8-10岁,分别。动物的平均精子运动性范围为57.66±2.60%至70.17±0.22%。从动物的尾骨区域获得血液样品。使用酶联免疫吸附测定法进行睾酮和脂联素分析。根据Williams方法使用碳醇品红-伊红染色评估精子形态。最后,睾酮和脂联素浓度之间的相关性,年龄,精子异常采用Pearson相关分析。
■研究结果表明,睾酮浓度与脂联素(-0.538)之间存在显着相关性(p<0.01),年龄(-0.588),精子形态异常(-0.912)。此外,他们发现8-10岁的公牛的睾酮浓度较低,在21.89±4.56ng/mL,与4-5岁的公牛相比,36.15±1.29ng/mL,6-7年,35.16±5.39ng/mL。研究结果还显示脂联素浓度与年龄(0.529)和精子异常(0.506)之间呈正相关。睾酮浓度的增加与脂联素浓度成反比(-0.538)。此外,异常精子的平均数量随着年龄的增加而增加:4-5岁组的3.82±0.33%,在6-7岁和8-10岁的组中,分别为4.40±0.72%和10.20±1.97%,分别。
■研究数据表明,睾酮浓度下降,高脂联素浓度,随着年龄的增长,公牛异常精子的增加。
UNASSIGNED: Capacity for sperm production is affected by age, which is related to the morphology of sperm abnormalities and can affect fertility. The aim of this study was to evaluate the relationship between age and concentrations of testosterone and adiponectin with sperm abnormalities in Simmental bulls.
UNASSIGNED: The study used 11 bulls, separated into three groups. The first group consisted of five bulls aged 4-5 years, and the second and third groups each consisted of three bulls, aged 6-7 and 8-10 years, respectively. The average sperm motility of the animals ranged from 57.66±2.60% to 70.17±0.22%. Blood samples were obtained from the coccygeal region of the animals. Testosterone and adiponectin analysis was performed using the enzyme-linked immunosorbent assay method. Sperm morphology was evaluated using carbol fuchsin-eosin staining according to the Williams method. Finally, correlations between testosterone and adiponectin concentrations, age, and sperm abnormalities were analyzed using Pearson\'s correlation analysis.
UNASSIGNED: The findings revealed a significant correlation (p<0.01) between the concentrations of testosterone and adiponectin (-0.538), age (-0.588), and abnormal sperm morphology (-0.912). Moreover, they revealed that the concentration of testosterone in the bulls aged 8-10 years was lower, at 21.89±4.56 ng/mL, compared to that in the bulls aged 4-5 years, at 36.15±1.29 ng/mL, and 6-7 years, at 35.16±5.39 ng/mL. The findings also revealed a positive correlation between adiponectin concentration and age (0.529) and sperm abnormalities (0.506). The increase in testosterone concentration was inversely related to the adiponectin concentration (-0.538). Moreover, the mean amount of abnormal sperm increased with increasing age: 3.82±0.33% in the group aged 4-5 years, and 4.40±0.72% and 10.20±1.97% in the groups aged 6-7 years and 8-10 years, respectively.
UNASSIGNED: The study data indicate that there is a decrease in testosterone concentration, a high adiponectin concentration, and an increase in abnormal sperm with increasing age in bulls.