■高睾丸温度导致雄性不育,影响哺乳动物精子发生。高睾丸温度影响精子活力,根据其大小和持续时间的形态和生育力。
■本研究的目的是研究热诱导的氧化应激和肉桂醛对Wistar大鼠睾丸结构和功能的影响。
■本实验中使用的大鼠是Wistar白化病大鼠。
■本研究每组6只动物。2.5-3个月大和275-300克的雄性Wistar白化病大鼠。(I)对照,(II)在41°C的密闭室中进行热应激(HS)14天,(III)用肉桂醛(CA)50mg/kg体重进行HS14天。(四)单独使用CA。研究结束后,动物被安乐死,并采集精子计数的测试样本,形态学,苏木精和伊红染色正常细胞形态,抗氧化剂和DNA完整性评估。
使用单向和双向ANOVA检验对数据进行统计学分析,以进行组间比较。
■应激组精子数量明显减少,精子形态较差。应激组的抗氧化能力远低于对照组。处于压力下的动物具有片段化的DNA。用肉桂醛治疗增加了整体抗氧化能力和精液参数,老鼠的行为最像对照组。
■CA恢复丙二醛水平,总抗氧化能力,暴露于实验性HS的大鼠的精子特征和减轻睾丸损伤。
UNASSIGNED: Male sterility results from high testicular temperatures, which affect mammalian spermatogenesis. High testicular temperatures affect sperm motility, morphology and fertility according to their magnitude and duration.
UNASSIGNED: The aim of the current study is to examine the effects of heat-induced oxidative stress and cinnamaldehyde on Wistar rat testicular structure and function.
UNASSIGNED: The rats used in this experiment were Wistar albino rats.
UNASSIGNED: This research has six animals per group. Male Wistar albino rats of 2.5-3 months old and 275-300 g. (I) control, (II) heat stress (HS) in a closed chamber at 41°C for 14 days and (III) HS with cinnamaldehyde (CA) 50 mg/kg body weight for 14 days. (IV) CA alone. After the study, the animals were euthanised, and test samples were taken for sperm count, morphology, haematoxylin and eosin stain for normal cellular morphology, antioxidants and DNA integrity assessments.
UNASSIGNED: The data were analysed statistically using one- and two-way ANOVA tests for comparisons between groups.
UNASSIGNED: The stress group had significantly lower sperm counts and poor sperm morphology. The stress group\'s antioxidant capacity is much lower than that of the control group. Animals under stress have fragmented DNA. Treatment with cinnamaldehyde increased overall antioxidant capacity and seminal parameters, and rats behaved most like controls.
UNASSIGNED: CA restores malondialdehyde levels, total antioxidant capacity, sperm characteristics and mitigates testicular damage in rats exposed to experimental HS.