背景:沙棘,俗称胡芦巴,它被用作香料。它有抗氧化剂,抗糖尿病,抗血脂和其他药理特性。
目的:本研究的目的是检测Trigonella-graecum(TF)的生物活性化合物Trigonelline(TG)在酒精中毒大鼠中的心脏保护活性。
方法:将年轻的wistar品系白化病大鼠分为5组,并按照实验方案进行治疗。抗氧化酶,超氧化物歧化酶(SOD),谷胱甘肽过氧化物酶(GPx),过氧化氢酶(CAT),谷胱甘肽还原酶(GR),谷胱甘肽(GSH),在所有实验组的心脏组织中估计丙二醛(MDA)水平。心脏标志物肌酸激酶-MB(CK-MB),肌钙蛋白T(TT),肌钙蛋白-I(TI),肌红蛋白(MG)和血清标志物丙氨酸转氨酶(AAT),估计天冬氨酸转氨酶(AST)和碱性磷酸酶(ALP)。自由基清除活性,如2,2-二苯基吡啶酰肼(DPPH),过氧化氢(H2O2)和羟基自由基估计在Trigonella-graecum的乙醇提取物中。
结果:SOD,CAT,GPx,GR,GSH活动耗尽,MDA,CK-MB,TT,TI,MG和AAT,AST,酒精中毒大鼠的ALP活性升高。对酒精大鼠补充Trigonelline30天,抗氧化酶升高,丙二醛耗尽,酒精中毒大鼠的心脏标志物和血清标志物。自由基清除试验还报道了三角菌具有自由基清除活性。此外,我们的组织病理学证据还证明,TG保护所有实验大鼠的心脏组织免受酒精诱导的毒性。
结论:我们的研究得出结论,TG可能对酒精和心肌梗死受试者有用。
BACKGROUND: Trigonella foenum-graecum, commonly known as fenugreek and it is used as a spice. It has antioxidant, anti-diabetic, antilipedemic and other pharmocological properties.
OBJECTIVE: The aim of the study was to detect the cardio protective activity of Trigonelline (TG) a bioactive compound of Trigonella foenum-graecum (TF) in alcohol intoxicated rats.
METHODS: The young wistar strain albino rats are divided in to 5 groups and treatment was given as per the experimental protocol. Antioxidant enzymes, superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT), glutathione reductase (GR), glutathione (GSH), malondialdehyde (MDA) levels are estimated in cardiac tissue of all experimental groups. Cardiac markers creatine kinase-MB (CK-MB), troponin-T (TT), troponin-I (TI), myoglobin (MG) and serum markers alanine transaminase (AAT), aspartate transaminase (AST) and alkaline phosphatase (ALP) are estimated. Free radical scavenging activities like 2,2-diphenylpicrylhydrazyl (DPPH), hydrogen peroxide (H2O2) and hydroxyl radical are estimated in ethanolic extract of Trigonella foenum-graecum.
RESULTS: SOD, CAT, GPx, GR, GSH activities are depleted and MDA, CK-MB, TT, TI, MG and AAT, AST, ALP activities are elevated in alcohol intoxicated rats. Trigonelline supplementation to alcoholic rats for 30 days elevated antioxidant enzymes, depleted MDA, cardiac markers and serum markers in alcohol intoxicated rats. Free radical scavenging assay also reported that Trigonella foenum-graecum possess free radical scavenging activity. Furthermore, our histopathological evidence also proved that TG protected the cardiac tissue from alcohol induced toxicity in all the experimental rats.
CONCLUSIONS: Our study concluded that TG may be useful to the alcoholic and myocardial infarction subjects.