Abstract：
Byrsonima Rich. is one of the largest genera of the Malpighiaceae family with 97 species occurrence in Brazil and multiple potentialities, including pharmaceutical and food industries. In this study, 17 microsatellite markers characterized in Byrsonima cydoniifolia were tested for seven related taxa, all species are native to Brazil and four are endemic. Genomic DNA was extracted from leaves tissues and 17 microsatellite markers were used to cross-amplification of microsatellite regions. Polymorphism and genetic diversity were evaluated for B. intermedia, B. verbascifolia, B. laxiflora, B. subterranea, B. umbellata, B. linearifolia. from 16 individuals and for B. viminifolia from 14 individuals. Transferred microsatellite markers panels ranged from 11 (64.8%) in B. viminifolia to 6 (35.2%) in B. umbellata. The total number of alleles per locus ranged from 5 (B. linearifolia) to 8 (B. subterranea) alleles. B. umbellata showed lower values of observed and expected heterozygosity (HO = 0.312; HE = 0.436) and B. subterranea presented the highest values (HO = 0.687; HE = 0.778). A greater number of microsatellite markers should be developed for B. umbellata. The microsatellite marker panels transferred to the species B. intermedia, B. verbascifolia, B. laxiflora, B. subterranea, B. viminifolia and B. linearifolia are very informative, with a high combined probability of exclusion of paternity (Q ≥ 0.976) and the low combined probability of identity (I ≤ 9.91 × 10-6), potentially suitable for future genetic-population studies, supporting strategies for maintaining the genetic diversity and for exploration of Byrsonima species as genetic resources.
摘要：
ByrsonimaRich.是Malpighiaceae家族中最大的属之一，在巴西有97种，具有多种潜力，包括制药和食品工业。在这项研究中,对Byrsonimacydoniifolia中的17个微卫星标记进行了7个相关分类群的测试，所有物种都原产于巴西，其中四个是特有的。从叶片组织中提取基因组DNA，并使用17个微卫星标记对微卫星区域进行交叉扩增。对中间芽孢杆菌的多态性和遗传多样性进行了评估，B.verbascifolia，B.laxiflora,B.地下，B.umbellata,B.线性化。来自16个个体和来自14个个体的B.viminifolia。转移的微卫星标记物组范围从B.viminifolia中的11个（64.8％）到B.umbellata中的6个（35.2％）。每个基因座的等位基因总数范围为5(B.线性化)到8(B.地下）等位基因。伞形芽孢杆菌显示观察到的和预期的杂合性值较低（HO=0.312；HE=0.436），地下芽孢杆菌显示出最高值（HO=0.687；HE=0.778）。应该为B.umbellata开发更多的微卫星标记。微卫星标记板转移到中间芽孢杆菌，B.verbascifolia，B.laxiflora,B.地下，B.viminifolia和B.linearifolia是非常有用的，具有较高的排除亲子关系的组合概率（Q≥0.976）和较低的同一性组合概率（I≤9.91×10-6），可能适合未来的遗传群体研究，支持维持遗传多样性和探索Byrsonima物种作为遗传资源的战略。
