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Abstract：
Brucellosis is an infectious zoonotic bacterial disease of humans and other animals. In the Republic of South Africa (RSA), animal brucellosis is widespread and the current available data on the prevalence of this disease rely solely on serological testing. The primary limitation of brucellosis serology is the lack of discriminatory powers to differentiate between Brucella species and biovars as well as the cross-reactivity observed with other Gram-negative bacteria.
The aim of this study was to conduct a retrospective laboratory-based survey on Brucella species and biovars isolated from various animal species in SA between 2008 and 2018.
The isolation of Brucella species and biovar typing was performed using conventional microbiological techniques.
A total of 963 strains of Brucella species were included in this study with a frequency of detection for B. abortus (n = 883; 91.6%) followed by B. melitensis (n = 42; 4.4%), B. ovis (n = 29; 3.0%) and B. canis (n = 9; 0.9%). Of the 883 strains of B. abortus, 90.1% were typed as B. abortus biovar-1 while 5.7% as B. abortus biovar-2, and 3.3% and 0.5% were B. abortus S19 and B. abortus RB51 vaccine strains, respectively. Among the 42 B. melitensis strains, 71.4% were reported as B. melitensis biovar-1 and 26.2% as B. melitensis biovar-3 while 2.4% was B. melitensis biovar-2.
A retrospective study, such as this one, provides useful information that can be critical in formulating policies and strategies for the control and eradication of brucellosis in animal populations in RSA.
The aim of this study was to conduct a retrospective laboratory-based survey on Brucella species and biovars isolated from various animal species in SA between 2008 and 2018.
The isolation of Brucella species and biovar typing was performed using conventional microbiological techniques.
A total of 963 strains of Brucella species were included in this study with a frequency of detection for B. abortus (n = 883; 91.6%) followed by B. melitensis (n = 42; 4.4%), B. ovis (n = 29; 3.0%) and B. canis (n = 9; 0.9%). Of the 883 strains of B. abortus, 90.1% were typed as B. abortus biovar-1 while 5.7% as B. abortus biovar-2, and 3.3% and 0.5% were B. abortus S19 and B. abortus RB51 vaccine strains, respectively. Among the 42 B. melitensis strains, 71.4% were reported as B. melitensis biovar-1 and 26.2% as B. melitensis biovar-3 while 2.4% was B. melitensis biovar-2.
A retrospective study, such as this one, provides useful information that can be critical in formulating policies and strategies for the control and eradication of brucellosis in animal populations in RSA.
摘要：
布鲁氏菌病是人类和其他动物的传染性人畜共患细菌性疾病。南非共和国（RSA）动物布鲁氏菌病很普遍，目前关于这种疾病流行的现有数据仅依赖于血清学检测。布鲁氏菌病血清学的主要局限性是缺乏区分布鲁氏菌物种和生物变体的歧视性能力，以及与其他革兰氏阴性细菌观察到的交叉反应性。
这项研究的目的是对2008年至2018年间从SA的各种动物物种中分离出的布鲁氏菌物种和生物谷进行回顾性实验室调查。
使用常规微生物学技术进行布鲁氏菌种的分离和biovar分型。
本研究共纳入963株布氏杆菌菌株，其检测频率为流产芽孢杆菌（n=883；91.6％），其次是B.melitensis（n=42；4.4％），B.Ovis(n=29;3.0%)和B.canis(n=9;0.9%)。在883株流产芽孢杆菌中，90.1％的类型为B.abortusbiovar-1，而5.7％的类型为B.abortusbiovar-2，3.3％和0.5％的类型为B.abortusS19和B.abortusRB51疫苗株，分别。在42株B.melitensis菌株中，71.4％被报告为B.melitensisbiovar-1，26.2％被报告为B.melitensisbiovar-3，而2.4％被报告为B.melitensisbiovar-2。
一项回顾性研究，比如这个,提供了有用的信息，这些信息对于制定RSA中动物种群中布鲁氏菌病的控制和根除政策和策略至关重要。
这项研究的目的是对2008年至2018年间从SA的各种动物物种中分离出的布鲁氏菌物种和生物谷进行回顾性实验室调查。
使用常规微生物学技术进行布鲁氏菌种的分离和biovar分型。
本研究共纳入963株布氏杆菌菌株，其检测频率为流产芽孢杆菌（n=883；91.6％），其次是B.melitensis（n=42；4.4％），B.Ovis(n=29;3.0%)和B.canis(n=9;0.9%)。在883株流产芽孢杆菌中，90.1％的类型为B.abortusbiovar-1，而5.7％的类型为B.abortusbiovar-2，3.3％和0.5％的类型为B.abortusS19和B.abortusRB51疫苗株，分别。在42株B.melitensis菌株中，71.4％被报告为B.melitensisbiovar-1，26.2％被报告为B.melitensisbiovar-3，而2.4％被报告为B.melitensisbiovar-2。
一项回顾性研究，比如这个,提供了有用的信息，这些信息对于制定RSA中动物种群中布鲁氏菌病的控制和根除政策和策略至关重要。
