背景:多杀性巴氏杆菌是全世界猪的重大经济损失的原因。在临床上患病的猪中,大多数多杀性疟原虫分离株的特征是多杀性疟原虫亚种,biovar2或3和囊型A或D;然而,关于亚种的信息很少,biovars,以及韩国其他荚膜型多杀性疟原虫分离株。这里,我们使用表型和基因型特征以及与最低抑制浓度相关的数据,提供了涵盖韩国肺炎猪中多杀性疟原虫延长时间段的信息.
结果:2008年至2016年期间,多杀性疟原虫的总体患病率为16.8%(240/1430),85%的多杀性疟原虫分离株(204/240)与其他呼吸道病原体共感染。在240个分离株中,本研究中包括166个;所有这些多杀性疟原虫分离株都被表征为多杀性亚种,并且最普遍的表型由biovar3(68.7%;n=114)和荚膜A型(69.9%;n=116)表示。此外,确定了三个囊状F型分离株,这代表了韩国此类分离株的第一份报告。所有Biovar1和2分离株均为囊型F和A,分别。毒力相关基因分布是可变的;所有荚膜A型和D型分离株分别含有pmHAS和hsf-1(P<0.001),F型(biovar1)与hsf-1(P<0.05)和pfhA(P<0.01)显着相关,Biovar2与pfhA和pmHAS高度相关,Biovar3与hsf-1、pmHAS、和HGBB(P<0.001),而biovar13仅与hgbB相关(P<0.05)。发现最高的耐药率是土霉素(63.3%),其次是氟苯尼考(16.3%)。
结论:P.多杀亚种多杀亚种,Biovar3,而荚膜A型是本研究中最普遍的分离株,我们的发现表明在韩国出现了囊型F。此外,由于确定的高耐药率,因此需要谨慎使用土霉素和氟苯尼考。需要进一步的研究来持续监测抗菌素耐药性,患病率,和多杀性疟原虫的流行病学特征,需要实验性感染模型来定义荚膜F的致病性。
BACKGROUND: Pasteurella multocida is responsible for significant economic losses in pigs worldwide. In clinically diseased pigs, most P. multocida isolates are characterised as subspecies multocida,
biovar 2 or 3 and capsular type A or D; however, there is little information regarding subspecies, biovars, and other capsular types of P. multocida isolates in Korea. Here, we provided information covering an extended time period regarding P. multocida in pigs with pneumonia in Korea using phenotypic and genotypic characterisations and data associated with the minimum inhibitory concentrations.
RESULTS: The overall prevalence of P. multocida between 2008 and 2016 was 16.8% (240/1430), with 85% of the P. multocida isolates (204/240) coinfected with other respiratory pathogens. Of the 240 isolates, 166 were included in this study; all of these P. multocida isolates were characterised as subspecies multocida and the most prevalent phenotypes were represented by biovar 3 (68.7%; n = 114) and capsular type A (69.9%; n = 116). Additionally, three capsular type F isolates were identified, with this representing the first report of such isolates in Korea. All
biovar 1 and 2 isolates were capsular types F and A, respectively. The virulence-associated gene distribution was variable; all capsular type A and D isolates harboured pmHAS and hsf-1, respectively (P < 0.001), with type F (biovar 1) significantly correlated with hsf-1 (P < 0.05) and pfhA (P < 0.01),
biovar 2 highly associated with pfhA and pmHAS, and biovar 3 significantly correlated with hsf-1, pmHAS, and hgbB (P < 0.001), whereas
biovar 13 was related only to hgbB (P < 0.05). The highest resistance rate was found to be to oxytetracycline (63.3%), followed by florfenicol (16.3%).
CONCLUSIONS: P. multocida subspecies multocida,
biovar 3, and capsular type A was the most prevalent isolate in this study, and our findings indicated the emergence of capsular type F in Korea. Moreover, prudent use of oxytetracycline and florfenicol is required because of the identified high resistance rates. Further studies are required for continuous monitoring of the antimicrobial resistance, prevalence, and epidemiological characterisation of P. multocida, and experimental infection models are needed to define the pathogenicity of capsular type F.