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Abstract：
The detection of water contamination with Legionella pneumophila is of critical importance to manufacturers of water processing equipment and public health entities dealing with water networks and distribution systems. Detection methods based on polymerase chain reaction or biosensor technologies require preconcentration steps to achieve attractive sensitivity levels. Preconcentration must also be included in protocols of automated collection of water samples by systems designed for quasi-continuous monitoring of remotely located water reservoirs for the presence of L. pneumophila. We designed and characterized a water sampling module for filtration and backwashing intended for analysis of low-to-medium contaminated water, typically with L. pneumophila bacteria not exceeding 50 colony-forming units per milliliter. The concentration factors of 10× and 21× were achieved with 0.22 and 0.45 µm filters, respectively, for samples of bacteria prepared in clean saline solutions. However, a 5× concentration factor was achieved with 0.45 µm filters for a heavily contaminated or turbid water typical of some industrial water samples.
摘要：
检测嗜肺军团菌的水污染对于水处理设备制造商和处理水网络和分配系统的公共卫生实体至关重要。基于聚合酶链反应或生物传感器技术的检测方法需要预浓缩步骤以实现有吸引力的灵敏度水平。预浓缩还必须包括在自动收集水样的协议中，该协议通过设计用于准连续监测远程水库中是否存在肺炎杆菌的系统进行。我们设计并表征了用于过滤和反冲洗的水采样模块，旨在分析中低污染水，通常与嗜肺乳杆菌细菌不超过每毫升50菌落形成单位。使用0.22和0.45µm过滤器实现了10×和21×的浓度因子，分别,在干净的盐溶液中制备的细菌样品。然而,对于某些工业用水样品中典型的严重污染或浑浊的水，使用0.45µm的过滤器可以获得5倍的浓缩系数。
