人类多能干细胞的定向分化用于产生高阶肾脏类器官。
关键词: 2D Monolayer 3D Organotypic culture Differentiation Flow cytometry Human pluripotent stem cells Immunocytochemistry Intermediate mesoderm Kidney organoid Nephron progenitor cells Nephrons Primitive streak
Mesh : Cell Culture Techniques Cell Differentiation Cells, Cultured Flow Cytometry Fluorescent Antibody Technique Gene Expression Regulation, Developmental Humans Kidney / cytology physiology Microscopy, Fluorescence Morphogenesis Organoids Pluripotent Stem Cells / physiology Signal Transduction Time Factors Tissue Engineering
来 源: DOI:10.1007/978-1-0716-1174-6_12