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Abstract：
Antimicrobial peptide (AMP) is a crucial component of the innate immune system in crustaceans. In mud crab, Scylla paramamosain, a commercially important species, a glycine-rich antimicrobial peptide (Spgly-AMP) gene was newly identified and putatively encoded a 26aa signal peptide and 37aa mature peptide. To understand the function of Spgly-AMP, the expression profile of Spgly-amp gene was characterized, which showed Spgly-amp was expressed widely in most tissues of adult crabs with the highest expression level in hemocytes. After Vibrio parahaemolyticus, PGN, or Poly I:C stimulations, the expression level of Spgly-amp was significantly up-regulated in the hemocytes. In antimicrobial assays, chemically synthesized Spgly-AMP peptides exhibited strong antibacterial activities against both Gram-positive and Gram-negative bacteria and high thermal stability after high-temperature heating. These findings in the present study verified the importance of the Spgly-AMP in defense of pathogenic bacteria infection in the mud crab and provided a promising candidate of antimicrobial agents in the crab aquaculture.
摘要：
抗菌肽（AMP）是甲壳类动物先天免疫系统的重要组成部分。在泥蟹中，Scyllaparamamosain,一个商业上重要的物种，新鉴定出富含甘氨酸的抗菌肽（Spgly-AMP）基因，并推定编码26aa信号肽和37aa成熟肽。要了解Spgly-AMP的功能，Spgly-amp基因的表达谱进行了表征，这表明Spgly-amp在成年蟹的大多数组织中广泛表达，在血细胞中表达水平最高。副溶血性弧菌之后,PGN,或者PolyI:C刺激，Spgly-amp的表达水平在血细胞中显著上调。在抗菌试验中，化学合成的Spgly-AMP肽对革兰氏阳性菌和革兰氏阴性菌均具有很强的抗菌活性，高温加热后具有很高的热稳定性。本研究中的这些发现证实了Spgly-AMP在防御泥蟹病原菌感染中的重要性，并为蟹水产养殖中的抗微生物剂提供了有希望的候选药物。
